Human Mouse Rat
ICC IHC WB
Human Synaptotagmin-1 is a 47,442 dalton protein (422 amino acids) associated with pre-synaptic terminals of the PNS and CNS. Synaptotagmin-1 binds calcium and initiates the fusion of vesicles with the pre-synaptic membrane. This protein also is believed to be the target of proteolytic activity associated with Botulinum neurotoxin A.
Chickens were immunized with four synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Synaptotagmin-1 gene product, but are shared between the human (NP_005630, NCBI) and mouse (NP_033332, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentra-tions of the eluates adjusted to 400 ug/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Synaptotagmin-1 (Synaptotagmin I) (SytI) (p65)
SYT1 SVP65 SYT
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Citations and References
- Salehi P, Ge MX, Gundimeda U, Michelle Baum L, Lael Cantu H, Lavinsky J, Tao L, Myint A, Cruz C, Wang J, Nikolakopoulou AM, Abdala C, Kelley MW, Ohyama T, Coate TM, Friedman RA. (2017), 'Role of Neuropilin-1/Semaphorin-3A signaling in the functional and morphological integrity of the cochlea.' Plos Genetics. 10.1371/journal.pgen.1007048.
- Harley RJ, Murdy JP, Wang Z, Kelly MC, Ropp TF, Park SH, Maness PF, Manis PB, Coate TM. (2018), 'Neuronal Cell Adhesion Molecule (NrCAM) is Expressed by Sensory Cells in the Cochlea and is Necessary for Proper Cochlear Innervation and Sensory Domain Patterning During Development .' Developmental Dynamics . 10.1002/dvdy.24629.
- Weston MR, Mindell JA. (2018), 'Characterizing chloride-dependent acidification in brain Clathrin-coated vesicles.' Biochemistry and Cell Biology. 10.1139/bcb-2018-0142.