Products: Primary Antibodies

Anti-S-tag (N56/9)


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
73-352		 Option
TC Supernatant		 Volume
5 mL		 Concentration
Lot dependent		 Price
$275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ELISA WB

Immunogen:

Identified as off-target monoclonal antibody in screen for anti-FGF14 NeuroMabs (immunogen was fusion protein amino acids 1-252, of full-length mouse FGF14b). Target identified as S-tag epitope (KETAAAKFERQHMDS, a 15 amino acid sequence derived from the pancreatic ribonuclease A and now frequently used as a protein tag) by ELISA and immunoblot against other S-tag fusion proteins.

Protein Name(s):

Ribonuclease pancreatic (EC 3.1.27.5) (RNase 1) (RNase A)

Gene Name(s):

RNASE1 RNS1

Antibody Registry ID:

TC Supernatant: AB_2315922

Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.