Identified as off-target monoclonal antibody in screen for anti-FGF14 NeuroMabs (immunogen was fusion protein amino acids 1-252, of full-length mouse FGF14b). Target identified as S-tag epitope (KETAAAKFERQHMDS, a 15 amino acid sequence derived from the pancreatic ribonuclease A and now frequently used as a protein tag) by ELISA and immunoblot against other S-tag fusion proteins.
Ribonuclease pancreatic (EC 220.127.116.11) (RNase 1) (RNase A)
TC Supernatant: AB_2315922
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.