Regular vials contain 1000 µL of this antibody mixture; Sampler vials contain 200 µL of this mixture.
Human Mouse Rat
Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The sequence of the synthetic peptide was shared between the human and mouse gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentration of the eluate adjusted to 100 µg/ml, and the preparation was filter sterilized.
Myelin proteolipid protein (PLP) (Lipophilin)
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Citations and References
- Lin JP, Mironova YA, Shrager P, Giger RJ. (2017), 'LRP1 regulates peroxisome biogenesis and cholesterol homeostasis in oligodendrocytes and is required for proper CNS myelin development and repair.' eLife. 10.7554/eLife.30498.001.