Products: Primary Antibodies

Anti-Prostatic Acid Phosphatase (PAP) – A Pain System Marker Antibody

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Product Summary:

Recombinant mouse PAP protein was expressed in bacteria. Antibodies against this protein were prepared by injecting purified recombinant PAP into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. These antibodies have been validated with human, mouse and rat tissues.


Brand Catalog # Option Volume Concentration Price
Aves Labs Logo
Catalog #
200 µL
10 mg/mL

Validation Images

Product Details

Antibody Type:


Host Species:


Species Reactivity:

Human Mouse Rat

WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot


Target Description:

Mouse Prostatic Acid Phosphatase (PAP) is a 43,698 dalton protein (381 amino acids; NCBI accession number AAF23171) associated with prostatic cancer cells, as well as primary afferent sensory neurons involved in the pain pathway. This protein is an enzyme that dephosphorylates adenosine monophosphate (AMP) in the dorsal horn gray matter of the spinal cord, generating free adenosine. Injections of PAP into the dorsal horn of experimental mice has been shown to decrease pain perception by acting in an antinociceptive, antihyperalgesic, and antiallodynic fashion.


Chickens were immunized with recombinant mouse ProstaticAcid Phosphatase protein. After repeated injections, immune eggs were collected from laying hens, from which IgY antibody were prepared (“anti-PAP IgY fraction”). Some of this antibody was further purified using an agarose matrix to which the PAP protein was convalently attached (“Affinity-purified anti-PAP”). The final preparation in the accompanying vial contains 10 mg/mLof the “anti-PAP IgY fraction” supplemented with 20 mg/mLof the “affinity-purified anti-PAP” plus 50% (v/v) glycerol (to prevent freezing at –20˚C). Finally, this antibody preparation was filter-sterilized (0.45 mm) and 200 ul aliquots prepared.

Protein Name(s):

Prostatic acid phosphatase (PAP) (EC (5'-nucleotidase) (5'-NT) (EC (Ecto-5'-nucleotidase) (Thiamine monophosphatase) (TMPase) [Cleaved into: PAPf39]

Gene Name(s):


Antibody Registry ID:


Antibody Validation and Application Notes

This antibody has been validated using the following assays:
Molecular Weight:

45 kDa

Western Dilution Range:


IHC Dilution Range:


Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Antibodies were analyzed using immunohistochemistry with tissue sections through a 10%-formalin fixed adult mouse. Sections were examined for PAPpositive dorsal root ganglion sensory neurons. Secondary antibodies (fluorescein-labeled goat anti-chicken IgY, Aves Cat. #F-1004) were used at a concentration of 1:500.

Citations and References