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Products: Primary Antibodies

Anti-Prostatic Acid Phosphatase (PAP) – A Pain System Marker


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
PAP
Volume
Regular and sample vials contain 200 µL of this antibody mixture
Concentration
10.0 mg/ml
Price
$245.00

Validation Images


Overview

Brand:

Aves Labs

Antibody Type:

Polyclonal

Host Species:

Chicken

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

IHC WB

Immunogen:

Chickens were immunized with recombinant mouse ProstaticAcid Phosphatase protein. After repeated injections, immune eggs were collected from laying hens, from which IgY antibody were prepared (“anti-PAP IgY fraction”). Some of this antibody was further purified using an agarose matrix to which the PAP protein was convalently attached (“Affinity-purified anti-PAP”). The final preparation in the accompanying vial contains 10 mg/ml of the “anti-PAP IgY fraction” supplemented with 20 mg/ml of the “affinity-purified anti-PAP” plus 50% (v/v) glycerol (to prevent freezing at –20˚C). Finally, this antibody preparation was filter-sterilized (0.45 mm) and 200 ul aliquots prepared.

Protein Name(s):

Prostatic acid phosphatase (PAP) (EC 3.1.3.2) (5'-nucleotidase) (5'-NT) (EC 3.1.3.5) (Ecto-5'-nucleotidase) (Thiamine monophosphatase) (TMPase) [Cleaved into: PAPf39]

Gene Name(s):

ACPP

Antibody Registry ID:

Default Title: AB_2313557


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Expected Banding Pattern:

45 kDa

Western Dilution Range:

1:1000-1:2000

IHC Dilution Range:

1:500-1:1000


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Antibodies were analyzed using immunohistochemistry with tissue sections through a 10%-formalin fixed adult mouse. Sections were examined for PAPpositive dorsal root ganglion sensory neurons. Secondary antibodies (fluorescein-labeled goat anti-chicken IgY, Aves Cat. #F-1004) were used at a concentration of 1:500.


Citations and References