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Products: Primary Antibodies

Anti-Prion Protein


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
PRN
Option
Mixture of IgY fraction (10 mg/ml) and affinity-purified antibodies (50 ug/ml)
Volume
200 ul
Concentration
10.0 mg/ml
Price
$245.00

Validation Images


Overview

Brand:

Aves Labs

Antibody Type:

Polyclonal

Host Species:

Chicken

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Immunogen:

Chickens were immunized with a recombinant fragment of the human prion protein emulsified with Freund’s complete and incomplete adjuvants.After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks.Affinity-purified antibodies were then prepared using an agarose column to which the recombinant protein fragments were covalently attached. Finally, affinity purified antibodies (50 ug/ml final concentration) were mixed with the IgY fraction (10 mg/ml final concentration) in a mixture containing glycerol (50% v/v) (to prevent freezing at -20°C) and 0.02% sodium azide.

Protein Name(s):

Major prion protein (PrP) (ASCR) (PrP27-30) (PrP33-35C) (CD antigen CD230)

Gene Name(s):

PRNP ALTPRP PRIP PRP

Antibody Registry ID:

Mixture of IgY fraction (10 mg/ml) and affinity-purified antibodies (50 ug/ml): AB_2313559


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
IHC Dilution Range:

1:1000-1:2000


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Antibodies were analyzed by immunocytochemistry (1:1000 dilution) using cells transfected with the prion protein cDNA.