Products: Primary Antibodies
Anti-Polyhistidine Anti-6XHIS (for Immunoprecipitation) Antibody Antibody
from Aves Labs
Our anti-6XHIS antibody recognizes the 6X-HIS tag with high titre and high specificity in immunoprecipitation applications.
PRODUCT OPTIONS
| Brand | Catalog # | Option | Volume | Concentration | Price | |
|---|---|---|---|---|---|---|
| Brand |
Catalog # HIS-IP-1010 |
Option Affinity-purified antibodies |
Volume 100 µL |
Concentration 1 mg/mL |
Price $335.00 |
Validation Images
Product Details
Polyclonal
Chicken
Affinity-purified antibodies
IP
Chickens were immunized with the 6X-Histidine peptide conjugated to keyhole limpet hemocyanin protein and emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using 6X-Histidine peptide conjugated to an agarose matrix. The antibody's concentration was then adjusted, mixed with glycerol and thimerosal, and filter-sterilized.
Affinity-purified antibodies: AB_2313519
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
Quality Control
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
This antibody preparation was analyzed by western blotting (at a dilution of 1:10000) using tissue extracts expressing a recombinant protein tagged with the 6X-HIS markerSecondary antibodies were HRP-labeled affinity-purified goat anti-chicken IgY (1:5000 dilution, Aves Labs Cat.# H-1004).