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Products: Primary Antibodies

Anti-Polyhistidine Anti-6XHIS (for Immunoprecipitation) Antibody


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
HIS-IP-1010
Option
Affinity-purified antibodies
Volume
100 µL
Concentration
1.0 mg/ml
Price
$315.00

Validation Images


Overview

Brand:

Aves Labs

Antibody Type:

Polyclonal

Host Species:

Chicken

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

IP

Immunogen:

Chickens were immunized with the 6X-Histidine peptide conjugated to keyhole limpet hemocyanin protein and emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using 6X-Histidine peptide conjugated to an agarose matrix. The antibody's concentration was then adjusted, mixed with glycerol and thimerosal, and filter-sterilized.

Antibody Registry ID:

Affinity-purified antibodies: AB_2313519


Antibody Validation and Application Notes


This antibody has been validated using the following assays:

Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

This antibody preparation was analyzed by western blotting (at a dilution of 1:10000) using tissue extracts expressing a recombinant protein tagged with the 6X-HIS markerSecondary antibodies were HRP-labeled affinity-purified goat anti-chicken IgY (1:5000 dilution, Aves Labs Cat.# H-1004).