Human Mouse Rat
ICC IHC WB
Human Perpherin is a 53,651 dalton protein (470 amino acids) expressed in all PNS neurons and some CNS neurons, including a subset of cortical neurons, hippocampal neurons, and ventral horn spinal cord motor neurons. At an ultrastructural level, peripherin immunoreactivity is associated with the neurofilament portion of the cytoskeleton, being particularly abundant in the cell body. Peripherin protein has been shown to undergo upregulation during periods of trophic stress in cultured neurons, and mutations of the peripherin gene have been associated with the neurodegenerative disease amyotrophic lateral sclerosis.
Chickens were immunized with full-length recombinant peripherin protein. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in the buffer described above, and then filter-sterilized.
Peripherin (Neurofilament 4)
PRPH NEF4 PRPH1
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:10000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Citations and References
- Baxter JC, Ramachandra R, Mayne DR, Elmslie KS. (2014), 'Functional expression of α7-nicotinic acetylcholine receptors by muscle afferent neurons.' Journal of Neurophysiology. 10.1152/jn.00035.2014.
- Ramachandra R, Elmslie KS. (2016), 'Voltage-dependent sodium (NaV) channels in group IV sensory afferents .' Molecular Pain. 10.1177/1744806916660721.
- Olson W, Abdus-Saboor I#, Cui L#, Burdge J, Raabe T, Ma M, Luo W. (2017), 'Sparse genetic tracing reveals regionally specific functional organization of mammalian nociceptors.' eLife. 10.7554/eLife.29507.001.
- Chamessian AG, Qadri YJ, Cummins M, Hendrickson M, Berta T, Buchheit T, Van de Ven T. (2017), '5-Hydroxymethylcytosine (5hmC) and Ten-eleven translocation 1–3 (TET1–3) proteins in the dorsal root ganglia of mouse: Expression and dynamic regulation in neuropathic pain.' Somatosensory & Motor Research. 10.1080/08990220.2017.1292237.