Products: Primary Antibodies

Anti-P-Zero Myelin Protein (Pz0) Antibody

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 Datasheet

Product Summary:

Two antipeptide antibodies were generated in chickens against sequences shared between the mouse (NP_032649) and human (NP_000521) gene products. Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
Aves Labs Logo
Catalog #
PZO
Volume
1000 µL
Concentration
200 µg/mL
Price
$399.00

Validation Images


Product Details

Antibody Type:

Polyclonal

Host Species:

Chicken

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Target Description:

Human Myelin Protein Zero (Pz0) is a 28,400 dalton protein (258 amino acids) found in myelin of the PNS, but not the CNS. Expressed in myelinating Schwann cells, Po serves as a homophilic adhesion molecule, allowing adjacent laminae of the myelin sheath to undergo compaction. Po is also a marker of a subpopulation of migrating neural crest cells. Mutations of the MPZ gene product in humans is responsible for Charcot-Marie-Tooth type 1B neuropathies (HMSN1B).

Immunogen:

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Po gene product, but are shared between the human (NP_000521, NCBI) and mouse (NP_032649, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 ug/ml. Finally, equal volumes of both of these affinitypurified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Protein Name(s):

Myelin protein P0 (Myelin peripheral protein) (MPP) (Myelin protein zero)

Gene Name(s):

MPZ

Antibody Registry ID:

AB_2313561


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Molecular Weight:

27 kDa

Western Dilution Range:

1:10000-1:20000

IHC Dilution Range:

1:2000-1:5000


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.


Citations and References