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Products: Primary Antibodies

Anti-P-Zero Myelin Protein (Pz0)


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
PZO
Volume
Regular vials contain 1000 µL of this antibody mixture; Sampler vials contain 200 µL of this mixture.
Concentration
200 µg/ml
Price
$399.00

Validation Images


Overview

Brand:

Aves Labs

Antibody Type:

Polyclonal

Host Species:

Chicken

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Immunogen:

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Po gene product, but are shared between the human (NP_000521, NCBI) and mouse (NP_032649, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 ug/ml. Finally, equal volumes of both of these affinitypurified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Protein Name(s):

Myelin protein P0 (Myelin peripheral protein) (MPP) (Myelin protein zero)

Gene Name(s):

MPZ

Antibody Registry ID:

Default Title: AB_2313561


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Molecular Weight:

27 kDa

Western Dilution Range:

1:10000-1:20000

IHC Dilution Range:

1:2000-1:5000


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.


Citations and References