Products: Primary Antibodies

Anti-NF-M (Neurofilament 160 kDa)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # NFM		Volume Regular vials contain 200 µL of this antibody mixture	Concentration 2.0 mg/ml	Price $245.00

Validation Images

Overview

Brand:

Aves Labs

Antibody Type:

Polyclonal

Host Species:

Chicken

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Immunogen:

NFM protein was purified from bovine spinal cords following the method of Delacourte et al. [“Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion” (1980). Biochem. J. 191(2): 543-546], followed by Prepcell purification (Bio-Rad). Antibodies were prepared by injecting purified NFM protein into laying hens. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in the buffer described above, and then filter-sterilized.

Protein Name(s):

Neurofilament medium polypeptide (NF-M) (160 kDa neurofilament protein) (Neurofilament 3) (Neurofilament triplet M protein)

Gene Name(s):

NEFM NEF3 NFM

Antibody Registry ID:

Default Title: AB_2313554

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Expected Banding Pattern:

150 kDa

Western Dilution Range:

1:1000-1:2000

IHC Dilution Range:

1:1000-1:2000

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:5000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Citations and References

Figlia G, Norrmén C, Pereira JA, Gerber D, Suter U (2017), 'Dual function of the PI3K-Akt-mTORC1 axis in myelination of the peripheral nervous system.' eLife. 10.7554/eLife.29241.
Arevalo-Martin A, Grassner L, Garcia-Ovejero D, Paniagua-Torija B, Barroso-Garcia G, Arandilla AG, Mach O, Turrero A, Vargas E, Alcobendas M, Rosell C, Alcaraz MA, Ceruelo S, Casado R, Talavera F, Palazón R, Sanchez-Blanco N, Maier D, Esclarin A, Molina-Holgado E (2018), 'Elevated Autoantibodies in Subacute Human Spinal Cord Injury Are Naturally Occurring Antibodies.' Frontiers in Immunology. 10.3389/fimmu.2018.02365.
Lee SJ, Oses-Prieto JA, Kawaguchi R, Sahoo PK, Kar AN, Rozenbaum M, Oliver D, Chand S, Ji H, Shtutman M, Miller-Randolph S, Taylor RJ, Fainzilber M, Coppola G, Burlingame AL, Twiss JL (2018), 'hnRNPs interacting with mRNA localization motifs define axonal RNA regulons.' Molecular & Cellular Proteomics. 10.1074/mcp.RA118.000603.
Hines TJ, Gao X, Sahu S, Lange MM, Turner JR, Twiss JL, Smith DS (2018), 'An Essential Postdevelopmental Role for Lis1 in Mice.' eNeuro. 10.1523/ENEURO.0350-17.2018.