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Products: Primary Antibodies

Anti-NF-H (Neurofilament 200 kDa) Antibody

Aves Labs Logo from Aves Labs

 Datasheet

Product Summary:

NFH protein was purified from bovine spinal cords following the method of Delacourte et al. ["Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion” (1980). Biochem. J. 191(2): 543-546], followed by Prepcell purification (Bio-Rad). Antibodies were prepared by injecting purified NFH protein into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. These antibodies have been found to cross-react with NFH protein from a variety of species, including human, mouse and rat.

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
Aves Labs Logo
Catalog #
NFH
Volume
200 µL
Concentration
1 mg/mL
Price
$245.00

Validation Images


Product Details

Antibody Type:

Polyclonal

Host Species:

Chicken

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Target Description:

Human Neurofilament Heavy Chain (NFH) is a 115,378 dalton protein (1072 amino acids) that forms part of the neuronal cytoskeleton. NFH is physically associated with 10 nm intermediate filaments and is said to be one of the "neurofilament triplet" proteins, although it probably serves more to stabilize the cytoskeleton rather than as an integral structural component. NFH immunoreactivity is found in neuronal somata, dendrites and axons.

Immunogen:

Chickens were immunized with NF-H protein purified from adult bovine brains. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in the buffer described above, and then filter-sterilized.

Protein Name(s):

Neurofilament heavy polypeptide (NF-H) (200 kDa neurofilament protein) (Neurofilament triplet H protein)

Gene Name(s):

NEFH KIAA0845 NFH

Antibody Registry ID:

AB_2313552


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Molecular Weight:

200-220 kDa

Western Dilution Range:

1:2000-1:5000

IHC Dilution Range:

1:2000-1:5000


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:50000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.


Citations and References