Products: Primary Antibodies

Anti-Neuron-Specific Enolase (NSE)


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
NSE		 Volume
Regular vials contain 1000 µL of this antibody mixture; Sampler vials contain 200 µL of this mixture.		 Concentration
200 µg/ml 		Price
$399.00

Validation Images

Overview

Brand:

Aves Labs

Antibody Type:

Polyclonal

Host Species:

Chicken

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Immunogen:

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the NSE-2 gene product, but are shared between the human (NP_001966, NCBI) and rat (AAA41119, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 ug/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filtersterilized.

Protein Name(s):

Gamma-enolase (EC 4.2.1.11) (2-phospho-D-glycerate hydro-lyase) (Enolase 2) (Neural enolase) (Neuron-specific enolase) (NSE)

Gene Name(s):

ENO2

Antibody Registry ID:

AB_2313555

Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Molecular Weight:

47 kDa

Western Dilution Range:

1:10000-1:20000

IHC Dilution Range:

1:10000-1:20000

Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:5000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Citations and References