Products: Primary Antibodies
Anti-Neuron-Specific Enolase (NSE) Antibody
from Aves Labs
Two antipeptide antibodies were generated in chickens against a sequence shared between the rat (AAA41119) and human (NP_001966) gene products. Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.
PRODUCT OPTIONS
| Brand | Catalog # | Option | Volume | Concentration | Price | |
|---|---|---|---|---|---|---|
| Brand |
Catalog # NSE |
Volume 1000 µL |
Concentration 200 µg/mL |
Price $399.00 |
Validation Images
Product Details
Polyclonal
Chicken
Human Mouse Rat
ICC IHC WB
Human NSE-2 (EC 4.2.1.11) is a 47,138 dalton protein (434 amino acids) expressed in neurons of the peripheral nervous system (PNS) and central nervous system (CNS). NSE-2 catalyzes the conversion of 2-phospho-D-glycerate into phosphoenol pyruvate and is an essential enzyme in energy metabolism in nervous tissues.
Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the NSE-2 gene product, but are shared between the human (NP_001966, NCBI) and rat (AAA41119, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 ug/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filtersterilized.
Gamma-enolase (EC 4.2.1.11) (2-phospho-D-glycerate hydro-lyase) (Enolase 2) (Neural enolase) (Neuron-specific enolase) (NSE)
ENO2
AB_2313555
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
47 kDa
1:10000-1:20000
1:10000-1:20000
Quality Control
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:5000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Citations and References
- Hegde S, Capell WR, Ibrahim BA, Klett J, Patel NS, Sougiannis AT, Kelly MP. (2016), 'Phosphodiesterase 11A (PDE11A), Enriched in Ventral Hippocampus Neurons, is Required for Consolidation of Social but not Nonsocial Memories in Mice.' Neuropsychopharmacology . 10.1038/npp.2016.106.