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Products: Primary Antibodies

Anti-Neu-N (Fox 3) Antibody

Aves Labs Logo from Aves Labs

 Datasheet

Product Summary:

Antipeptide antibody was purified from eggs collected from chickens injected with a KLH-conjugated peptide corresponding to a sequence shared between the mouse (NP_001020102) and human (NP_001076044) gene products. These antibodies have been validated with human, mouse and rat tissues.

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
Aves Labs Logo
Catalog #
NUN
Volume
1000 µL
Concentration
100 µg/mL
Price
$399.00

Validation Images


Product Details

Antibody Type:

Polyclonal

Host Species:

Chicken

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC

Target Description:

Human Neu-N (Fox 3) is a 33,873 dalton (312 amino acid) RNA-binding protein. This protein is found in the nuclei of virtually all post-mitotic central and peripheral neurons and has been used extensively as a general neuronal marker. In these studies, Neu-N (Fox 3) stains neuronal nuclei relatively uniformly, except in the region of the nucleolus, providing a distinctive donut (i.e., torus) shape. Since this antigen is not seen in the cytoplasm, it offers the advantage of being useful in co-immunostaining studies with rabbit and mouse antibodies against various cytoplasmic neuronal antigens.

Immunogen:

Hens were immunized with a synthetic peptide-keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to the Fox-3 gene product (also known as the Neu-N antigen), but was shared between the human NP_001076044, NCBI) and mouse (NP_001020102, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 µg/mL, and the preparation filter-sterilized.

Protein Name(s):

RNA binding protein fox-1 homolog 3 (Fox-1 homolog C) (Neuronal nuclei antigen) (NeuN antigen)

Gene Name(s):

RBFOX3

Antibody Registry ID:

AB_2313556


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Molecular Weight:

46, 48 kDa

IHC Dilution Range:

1:1000-1:2000


Citations and References