Products: Primary Antibodies
Anti-Netrin-1 Antibody
from Aves Labs
An antipeptide antibody was generated in chickens against a sequence shared between the mouse (O09118) and human (O95631) gene products. Antibodies were affinity-purified and the concentration adjusted to 100 µg/mL. These antibodies have been validated with human, mouse and rat tissues.
PRODUCT OPTIONS
| Brand | Catalog # | Option | Volume | Concentration | Price | |
|---|---|---|---|---|---|---|
| Brand |
Catalog # NET |
Volume 1000 µL |
Concentration 100 µg/mL |
Price $399.00 |
Validation Images
Product Details
Polyclonal
Chicken
Human Mouse Rat
ICC IHC
Murine netrin-1 precursor is a 67,768 dalton protein (604 amino acids) expressed by subpopulations of cells within the developing CNS, as well as in various non-neural tissues, including the gut, liver, heart, and prostate. In the embryonic CNS, netrin-1 is expressed by cells of the floor plate, and acts to attract axons of "commissural neurons" from the dorsal horn gray matter. Netrin-1 is also believed to play a role in the growth of motor axons in the PNS.
Chickens were immunized with a synthetic peptide-keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to the netrin-1 gene product, but was shared between the human (O_095631, NCBI) and mouse (O_09118, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 ug/ml, and the preparation filter-sterilized.
Netrin-1 (Epididymis tissue protein Li 131P)
NTN1 NTN1L
AB_2313551
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
Not Recommended
1:1000-1:2000
Quality Control
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.