Products: Primary Antibodies
PRODUCT OPTIONS
| Brand | Catalog # | Option | Volume | Concentration | Price | |
|---|---|---|---|---|---|---|
Brand |
Catalog # NES |
Volume Regular vials contain 1000 µL of this antibody mixture; Sampler vials contain 200 µL of this mixture. |
Concentration 300 µg/ml |
Price $399.00 |
Validation Images
Overview
Brand:
Aves Labs
Antibody Type:
Polyclonal
Host Species:
Chicken
Species Reactivity:
Mouse Rat
Applications:
WB
Western Blot
IHC
Immunohistochemistry
ICC
Immunocytochemistry
IP
Immunoprecipitation
AT
Array Tomography
EM
Electron Microscopy
CHIP
Chromatin Immunoprecipitation
DB
Dot Blot
ICC IHC WB
Immunogen:
Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the mouse nestin gene product (NM_NP_057910), but are shared with the rat (NM_012987, NCBI) protein sequence. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 ug/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filtersterilized.
Protein Name(s):
Nestin
Gene Name(s):
NES Nbla00170
Antibody Registry ID:
AB_2314882
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
Molecular Weight:
250 kDa
Western Dilution Range:
1:10000-1:20000
IHC Dilution Range:
1:1000-1:2000
Quality Control
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:20000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Citations and References
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