Products: Primary Antibodies

Anti-Nestin Antibody

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 Datasheet

Product Summary:

Three antipeptide antibodies were generated in chickens against a sequence in mouse (NP_057910) gene product. Antibodies were affinity-purified and mixed in equal concentration. These antibodies have been validated with human, mouse and rat tissues.

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
Aves Labs Logo
Catalog #
NES
Volume
1000 µL
Concentration
300 µg/mL
Price
$399.00

Validation Images


Product Details

Antibody Type:

Polyclonal

Host Species:

Chicken

Species Reactivity:

Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Target Description:

Mouse Nestin is a 206,994 dalton protein (1864 amino acids) expressed in neural crest cells, CNS neural stems cells, as well as a few non-neural cell types in the embryo, including cells within the pancreatic islets of Langerhans and the limb bud. Nestin is an intermediate filament-associated protein, and contributes to the cytoskeleton.

Immunogen:

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the mouse nestin gene product (NM_NP_057910), but are shared with the rat (NM_012987, NCBI) protein sequence. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 ug/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filtersterilized.

Protein Name(s):

Nestin

Gene Name(s):

NES Nbla00170

Antibody Registry ID:

AB_2314882


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Molecular Weight:

250 kDa

Western Dilution Range:

1:10000-1:20000

IHC Dilution Range:

1:1000-1:2000


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:20000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.


Citations and References