Nestin

Catalog #	Option	Volume	Concentration	Price*
Catalog # NES		Volume Regular vials contain 1000 µL of this antibody mixture; Sampler vials contain 200 µL of this mixture.	Concentration 300 µg/ml	Price $399.00

Validation Images

Overview

Brand:

Aves Labs

Antibody Type:

Polyclonal

Host Species:

Chicken

Species Reactivity:

Mouse Rat

Applications:

ICC IHC WB

Immunogen:

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the mouse nestin gene product (NM_NP_057910), but are shared with the rat (NM_012987, NCBI) protein sequence. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 ug/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filtersterilized.

Protein Name(s):

Nestin

Gene Name(s):

NES Nbla00170

Antibody Registry ID:

AB_2314882

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Molecular Weight:

250 kDa

Western Dilution Range:

1:10000-1:20000

IHC Dilution Range:

1:1000-1:2000

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:20000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Citations and References

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