Products: Primary Antibodies

Anti-Nav1.5 Na+ channel (N397/19)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 73-382	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC WB

Immunogen:

Fusion protein amino acids 424-658 (cytoplasmic loop between repeats I and II) of human Nav1.5 (also known as Voltage-gated sodium channel subunit alpha Nav1.5, Sodium channel protein type 5 or V subunit alpha, Sodium channel protein cardiac muscle subunit alpha, SCN5A and HH1, accession number Q14524)

Mouse: 88% identity (209/235 amino acids identical)
Rat: 87% identity (206/235 amino acids identical)
40% identity with Nav1.1 and other Nav1 channels

Cross Reactivity:

Does not cross-react with Nav1.4

Protein Name(s):

Sodium channel protein type 5 subunit alpha (HH1) (Sodium channel protein cardiac muscle subunit alpha) (Sodium channel protein type V subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.5)

Gene Name(s):

SCN5A

Antibody Registry ID:

TC Supernatant: AB_2336909

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

230 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.