Products: Primary Antibodies
Anti-Myelin Basic Protein (MBP) Antibody
from Aves Labs
An antipeptide antibody was generated in chickens against a sequence shared between the mouse (NP_034907) and human (NP_002376) gene products. Antibodies were affinity-purified and the concentration adjusted to 100 µg/mL.
PRODUCT OPTIONS
| Brand | Catalog # | Option | Volume | Concentration | Price | |
|---|---|---|---|---|---|---|
| Brand |
Catalog # MBP |
Volume 1000 µL |
Concentration 100 µg/mL |
Price $399.00 |
Validation Images
Product Details
Polyclonal
Chicken
Human Mouse Rat
ICC IHC WB
Human Myelin Basic Protein (MBP) is a 20,115 dalton protein (186 amino acids) found in myelin of the CNS and PNS. Expressed as an intracellular protein by oligodendrocytes and Schwann cells, MBP aids in the compaction and stability of myelin.
Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the MBP gene product shared between the human (NP_002376, NCBI) and mouse (NP_034907, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column. The concentrations of the eluates were then adjusted to 100 ug/ml, and the preparation was filter-sterilized.
Myelin basic protein (MBP) (Myelin A1 protein) (Myelin membrane encephalitogenic protein)
MBP
AB_2313550
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
Can recognize multiple bands between 14-22 kDa, corresponding to different MBP isoforms
1:1000-1:2000
1:1000-1:2000
Quality Control
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Citations and References
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