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Products: Primary Antibodies

Anti-Myelin Basic Protein (MBP)


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
MBP
Volume
Regular vials contain 1000 µL of this antibody mixture; Sampler vials contain 200 µL of this mixture.
Concentration
100 µg/ml
Price
$399.00

Validation Images


Overview

Brand:

Aves Labs

Antibody Type:

Polyclonal

Host Species:

Chicken

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Immunogen:

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the MBP gene product shared between the human (NP_002376, NCBI) and mouse (NP_034907, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column. The concentrations of the eluates were then adjusted to 100 ug/ml, and the preparation was filter-sterilized.

Protein Name(s):

Myelin basic protein (MBP) (Myelin A1 protein) (Myelin membrane encephalitogenic protein)

Gene Name(s):

MBP

Antibody Registry ID:

Default Title: AB_2313550


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Molecular Weight:

Can recognize multiple bands between 14-22 kDa, corresponding to different MBP isoforms

Western Dilution Range:

1:1000-1:2000

IHC Dilution Range:

1:1000-1:2000


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.


Citations and References