Products: Primary Antibodies

Anti-mCherry Antibody

Aves Labs Logo from Aves Labs

 Datasheet

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
Aves Labs Logo
Catalog #
MCHERRY-0020
Option
20 µg
Volume
100µL
Concentration
0.2mg/mL
Price
$119.00
Brand
Aves Labs Logo
Catalog #
MCHERRY-0100
Option
100 µg
Volume
500uL
Concentration
0.2mg/mL
Price
$410.00

Validation Images


Product Details

Antibody Type:

Polyclonal

Host Species:

Chicken

Form:

IgY Fraction

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Target Description:

mCherry is a red fluorescent protein derived from DsRed which is isolated from disc corals and related to GFP (green fluorescent protein) (Shaner et al., 2004). Fluorescent proteins are commonly used to tag proteins in order to localize their expression in both cultured cells and in vivo. Antibodies against fluorescent proteins such as mCherry are especially useful when amplification of the fluorescent signal is necessary or desired. Our chicken anti-mCherry antibody is a highly sensitive reagent for detection of mCherry by western blot, immunocytochemistry, and immunohistochemistry.

Immunogen:

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes the expected molecular weight band when used to perform Western blot on lysates from cells overexpressing mCherry and on purified recombinant mCherry and related red fluorescent proteins. This antibody shows no binding to GFP or other off-target cellular proteins.

Immunocytochemistry:

This antibody recognizes mCherry in mCherry-overexpressing COS cells but gives no discernible signal in mock-transfected cells.

Molecular Weight:

27 KDa for native monomer


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested by Western blot on an mCherry overexpression cell lysate.


Citations and References

    This highly-validated antibody is a newly-launched product. Be the first to cite usage of this product and receive a free antibody.*

    *Free antibody is provided to verified customer and does not include cost of shipping.