Chickens were immunized with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA) conjugated to keyhole limpet hemocyanin. After repeated injections, immune eggs were collected, from which the IgY fractions were prepared. These fractions were then affinity-purified using a peptide column, coupled to HRP, and filter-sterilized (0.45 µm).
Affinity Purified: AB_2313513
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Antibodies were analyzed using ELISA and western blot analyses. By ELISA, the antibodies were diluted 1:10,000 and gave an optical density of >1.0 in a 15 minute reaction using tetramethyl benzidine as the substrate against peptide conjugated to a different carrier protein. By western blot, the antibodies were diluted 1:1,000 and gave a prominent band.