Products: Primary Antibodies

Anti-HA Epitope Tag Antibody

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # ET-HA100	Option Affinity Purified	Volume 1.0 mL	Concentration 100 µg/ml	Price $245.00

Overview

Brand:

Aves Labs

Antibody Type:

Polyclonal

Host Species:

Chicken

Applications:

ELISA ICC IHC IP WB

Immunogen:

Chickens were immunized with a synthetic peptide containing the influenza hemagglutinin (HA) epitope (i.e., YPYDVPDYA) conjugated to keyhole limpet hemocyanin. The first injection included complete Freund’s adjuvant; subsequent injections included incomplete Freund’s adjuvant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were affinity-purified over a peptide column and then dialyzed against phosphate-buffered isotonic saline. Sodium azide was added and the antibodies were filter-sterilized (0.2 µm).

Antibody Registry ID:

Affinity Purified: AB_2313511

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Dilution Range:

1:5000

IHC Dilution Range:

1:500

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Antibodies were analyzed using ELISA and western blots using an N-terminal HA-tagged protein. Secondary antibodies used in these quality control assays were alkaline phosphatase- (1:1000 dilution) and HRP-labeled (1:3000 dilution) goat anti-chicken IgY antibodies (Aves Labs), respectively.