Products: Primary Antibodies

Anti-HA Epitope Tag Antibody

Aves Labs Logo from Aves Labs

 Datasheet

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
Aves Labs Logo		 Catalog #
ET-HA100		 Option
Affinity Purified		 Volume
1.0 mL		 Concentration
100 µg/ml		 Price
$255.00

Validation Images

Overview

Brand:

Aves Labs

Antibody Type:

Polyclonal

Host Species:

Chicken

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ELISA ICC IHC IP WB

Immunogen:

Chickens were immunized with a synthetic peptide containing the influenza hemagglutinin (HA) epitope (i.e., YPYDVPDYA) conjugated to keyhole limpet hemocyanin. The first injection included complete Freund’s adjuvant; subsequent injections included incomplete Freund’s adju­vant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were affinity-purified over a peptide column and then dialyzed against phosphate-buffered isotonic saline. Sodium azide was added and the antibod­ies were filter-sterilized (0.2 µm).

Antibody Registry ID:

Affinity Purified: AB_2313511

Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Dilution Range:

1:5000

IHC Dilution Range:

1:500

Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Antibodies were analyzed using ELISA and western blots using an N-terminal HA-tagged protein. Secondary antibodies used in these quality control assays were alkaline phosphatase- (1:1000 dilution) and HRP-labeled (1:3000 dilution) goat anti-chicken IgY antibodies (Aves Labs), respectively.