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Products: Primary Antibodies

Anti-Glutamate Receptor (Metabotropic) Type 5 (mGluR5)


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
ER5
Volume
Regular vials contain 1000 µL of this antibody mixture; Sampler vials contain 200 µL of this mixture.
Concentration
300 µg/ml
Price
$399.00

Validation Images


Overview

Brand:

Aves Labs

Antibody Type:

Polyclonal

Host Species:

Chicken

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC

Immunogen:

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the metabotropic glutamate receptor type 5 gene product, but are shared between the human (NP_001137303.1, NCBI) and mouse (Q3UVX5.2, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 ug/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Protein Name(s):

Metabotropic glutamate receptor 5 (mGluR5)

Gene Name(s):

GRM5 GPRC1E MGLUR5

Antibody Registry ID:

Default Title: AB_2313544


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
IHC Dilution Range:

1:1000-1:2000


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

This anti-peptide antibody mixture was analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.