Products: Primary Antibodies

Anti-GABA(A)R, Theta Antibody (L123/109)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-493	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-493	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human

Applications:

ICC IHC

Target Description:

Gamma-aminobutyric acid receptor subunit theta or GABA AR-Theta is encoded by the gene GABRQ. GABAA receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR theta is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR-theta is expressed in brain at the cell junction, synapse,and the postsynaptic cell membrane with high protein levels in the hypothalamus and dentate gyrus.

Immunogen:

Fusion protein amino acids 22-268 (extracellular N-terminus) of human GABA-A-R-Theta (also known as Gamma-aminobutyric acid receptor subunit theta, GABA(A) receptor subunit theta and GABRQ, accession number Q9UN88)

Protein Name(s):

GABA-A-R-Theta (Gamma-aminobutyric acid receptor subunit theta) (GABA(A) receptor subunit theta)

Gene Name(s):

GABRQ

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.