Products: Primary Antibodies

Anti-Foxi3 (N359/28)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 73-338	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse

Applications:

ICC WB

Immunogen:

Fusion protein amino acids 1-127 (N-terminus) of mouse Foxi3 (also known as Forkhead box protein I3, RCG56131 and rCG_56131, accession number E0CZH3) Rat: 94% identity (97/103 amino acids identical, with N-terminal 26 amino acid truncation) Human: 73% identity (86/117 amino acids identical, with N-terminal 26 amino acid truncation) <50% identity with Foxi1 and Foxi2

Protein Name(s):

Forkhead box I3

Gene Name(s):

Foxi3

Antibody Registry ID:

TC Supernatant: AB_2315836

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Expected Banding Pattern:

50 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.