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Products: Primary Antibodies

Anti-DYKDDDDK Epitope Tag/FLAG Antibody


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
ET-DY100
Option
Affinity Purified
Volume
1.0 mL
Concentration
100 µg/ml
Price
$245.00

Validation Images


Overview

Brand:

Aves Labs

Antibody Type:

Polyclonal

Host Species:

Chicken

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ELISA ICC IHC IP WB

Immunogen:

Chickens were immunized with a synthetic peptide containing the DYKDDDDK epitope conjugated to keyhole limpet hemocyanin (KLH). The first injection included complete Freund’s adjuvant; subsequent injections included incomplete Freund’s adjuvant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were then affinity-purified over a peptide column and dialyzed against phosphate-buffered isotonic saline. Sodium azide was added, the protein concentration was adjusted to 100 mg/ml, and the antibodies were filter-sterilized (0.2 µm).

Antibody Registry ID:

Affinity Purified: AB_2313510


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Dilution Range:

1:1000-1:2000

IHC Dilution Range:

1:500-1:1000


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Antibodies were analyzed using ELISA and western blots using an N-terminal DYKDDDDK-tagged protein. Secondary antibodies used in these quality control assays was an HRP-labeled (1:5000 dilution) goat anti-chicken IgY antibodies (Aves Labs).