Products: Primary Antibodies
Anti-CNPase (cyclic nucleotide phosphodiesterase) Antibody
from Aves Labs
This antipeptide antibody was generated in chickens against a sequence shared between the mouse (P16330) and human (NP_149124) gene products. The antibody was affinity-purified, and its concentration was adjusted to 100 µg/mL. This antibody has been validated with human, mouse and rat tissues.
PRODUCT OPTIONS
| Brand | Catalog # | Option | Volume | Concentration | Price | |
|---|---|---|---|---|---|---|
| Brand |
Catalog # CNP |
Volume 1000 µL |
Concentration 100 µg/mL |
Price $410.00 |
Validation Images
Product Details
Polyclonal
Chicken
Human Mouse Rat
ICC IHC WB
Murine CNPase (EC 3.1.4.37) is a 47,122 dalton protein (420 amino acids) found in myelin. This protein is expressed by oligodendrocytes of the CNS and by Schwann cells of the PNS.
Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the CNPase gene product shared between the human (NP_149124, NCBI) and mouse (P16330, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column. The concentrations of the eluates were then adjusted to 100 ug/ml, and the preparation was filter-sterilized.
2',3'-cyclic-nucleotide 3'-phosphodiesterase, CNP, CNPase, EC 3.1.4.37
CNP
AB_2313538
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
47 kDa
1:500-1:1000
1:500-1:1000
Quality Control
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Citations and References
- Beirowski B, Wong KM, Babetto E, Milbrandt J. (2017), 'mTORC1 promotes proliferation of immature Schwann cells and myelin growth of differentiated Schwann cells .' PNAS. 10.1073/pnas.1620761114.
- Lin JP, Mironova YA, Shrager P, Giger RJ. (2017), 'LRP1 regulates peroxisome biogenesis and cholesterol homeostasis in oligodendrocytes and is required for proper CNS myelin development and repair.' eLife. 10.7554/eLife.30498.001.