Human Mouse Rat
Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the Choline Acetyltransferase gene product shared between the human (P28329, NCBI) and mouse (Q03059, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column. The concentrations of the eluates were then adjusted to 100 ug/ml, and the preparation was filter-sterilized.
Choline O-acetyltransferase (CHOACTase) (ChAT) (Choline acetylase) (EC 184.108.40.206)
Default Title: AB_2313537
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.