ELISA ICC IHC IP WB
Chickens were immunized with a synthetic peptide containing the human c-myc epitope (i.e., EQKLISEEDL) conjugated to keyhole limpet hemocyanin. The first injection included complete Freund’s adjuvant; subsequent injections included incomplete Freund’s adjuvant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were affinity-purified over a peptide column and then dialyzed against phosphate-buffered isotonic saline. Sodium azide was added and the antibodies were filter-sterilized (0.2 µm).
Affinity Purified: AB_2313514
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Antibodies were analyzed using ELISA and western blots using an N-terminal FLAG®-tagged protein. Secondary antibodies used in these quality control assays were alkaline phosphatase- (1:1000 dilution) and HRP-labeled (1:3000 dilution) goat anti-chicken IgY antibodies (Aves Labs), respectively.
Citations and References
- Grimaldo L, Sandoval A, Garza-López E, Felix R. (2017), 'Involvement of Parkin in the ubiquitin proteasome system-mediated degradation of N-type voltage-gated Ca2+ channels.' Plos One. 10.1371/journal.pone.0185289.