Products: Primary Antibodies

Anti-Beta-Gal (Beta-Galactosidase, LacZ) Antibody


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PRODUCT OPTIONS

Catalog # Option Concentration Price*
Catalog #
BGL-1010
Option
100 µL
Concentration
10.0 mg/ml
Price
$115.00
Catalog #
BGL-1040
Option
400 µL
Concentration
10.0 mg/ml
Price
$245.00

Overview

Brand:

Aves Labs

Antibody Type:

Polyclonal

Host Species:

Chicken

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Immunogen:

Chickens were immunized with purified ß-Galactosidase (lacZ) protein emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using ß-Galactosidase conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 µg/ml) and purified IgY (10 mg/ml), and is filter-sterilized.

Antibody Registry ID:

100 µL: AB_2313508
400 µL: AB_2313507


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Dilution Range:

1:5000-1:10000

IHC Dilution Range:

1:2500-1:5000


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

This antibody preparation was analyzed by immunohistochemistry (at a dilution of 1:5000) using tissues infected with a virus bearing the LacZ gene. Secondary antibodies were fluorescein-labeled affinity-purified goat anti-chicken IgY (1:2000 dilution, Aves Labs Cat.# F-1005).