Immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with N449/73 (left) and N52A/42 (right) TC supe.
Vesicular neurotransmitter transporters sequester the transmitters into synaptic vesicles (Erickson et al., 1996). The vesicular monoamine transporter 2 (VMAT2) is responsible for catecholamine and serotonin storage in central synapses. Antibodies specific for VMAT have been used to monitor expression of the transporter during development and in aging and can be effectively used as a marker for monoamine terminals (Haycock et al., 2003; Witkovsky et al., 2005).
Fusion protein amino acids 42-132 (first lumenal domain) of mouse Slc18a2 (also known as Synaptic vesicular amine transporter, Monoamine transporter, Solute carrier family 18 member 2, Vesicular amine transporter 2, VAT2, Svmt, Svat and Vmat2, accession number Q66L40); Rat: 82% identity (75/92 amino acids identical); Human: 73% identity (67/92 amino acids identical); 30% identity with Slc18a1/VMAT1
Human, Mouse, Rat
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Does not cross-react with Slc18a1/VMAT1 or Slc18a3/VAChT
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.