Anti-LRRK2/Dardarin, C-Terminus Recombinant Chicken Chimeric mAb (N241A/34)

Name: Anti-LRRK2/Dardarin, C-Terminus Recombinant Chicken Chimeric mAb
Brand: Aves Labs
SKU: 78-253
Price: 470.0 USD
Availability: InStock

Our chicken Anti-Dardarin/LRRK2, C-terminus recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N241A/34. It detects drosophila, human, mouse, non-human primate, and rat Dardarin/LRRK2, C-terminus and is purified by affinity chromatography. It works in WB, IHC, ICC, IP, ELISA.

Representative section of formalin fixed, paraffin-embedded rat brain cerebral cortex showing cells stained by anti-LRRK2/Dardarin antibody. The sections were stained with Aves Labs anti-LRRK2 recombinant chicken monoclonal antibody (visualized in green, Cat No. 78-253). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei.

Western blotting of mock transfected and LRRK2-FLAG transfected COS-7 lysates with anti-LRRK2/Dardarin mouse Mab (Cat No.75-253) and Aves Labs anti-LRRK2/Dardarin recombinant chicken Mab (Cat No. 78-253) antibody. The mouse Mab and chicken Mab were detected with anti-mouse-HRP and anti-chicken-HRP, respectively. Aves Labs Recombinant Chicken anti-LRRK2 Mab recognizes exogenous LRRK2 with similar sensitivity as the anti-LRRK2 NeuroMab only in LRRK2-FLAG transfected COS-7 lysates at >200kDa.

Representative section of formalin fixed, paraffin-embedded rat brain cerebral cortex showing cells stained by anti-LRRK2/Dardarin antibody. The sections were stained with Aves Labs anti-LRRK2 recombinant chicken monoclonal antibody (visualized in green, Cat No. 78-253). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei.

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Species Reactivity

Drosophila, Human, Mouse, Non-Human Primate, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

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Data Sheet

Trial Size

SKU: 78-253

Volume: 100 µL

Variant

Price:

$470.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Dardarin/LRRK2, C-terminus

Scientific Background

LRRK2 (also known as PARK8) encodes a protein with 5 putative functional domains: an N-terminal leucine-rich repeat (LRR) domain, a Roc (Ras of complex protein) domain that shares sequence homology to the Ras-related GTPase superfamily, a COR (C-terminal of Roc) domain, a mitogen-activated protein kinase kinase kinase (MAPKKK) domain, and a C-terminal WD40 repeat domain. Mutation in this gene is one of the most common causes of inherited Parkinson disease (Gandhi et al., 2008). LRRK2 was originally identified as a putative disease-causing transcript (DKFZp434H2111) within a 2.6-Mb region encompassing a locus for Parkinson disease-8 (PARK8). Northern blot analysis detected a 9-kb mRNA transcript in all tissues tested, including brain. The authors named the protein product dardarin, derived from the Basque word dardara, meaning tremor. LRRK2/dardarin is also known to positively regulate autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway and together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. LRRK2/PARK8 is also known to regulate neuronal process morphology in the intact central nervous system (CNS) and play a role in synaptic vesicle trafficking.

Format

Purified by affinity chromatography

Concentration

1 mg/mL

Clonality

Recombinant

Clone

N241A/34

Isotype

IgY

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Gene Name

LRRK2 PARK8

Molecular Weight

>200 kDa

Immunogen

Fusion protein amino acids 970-2527 (C-terminus) of human LRRK2 (accession number Q5SS00) produced recombinantly in E. Coli.

Immunogen Species

Human

Species Reactivity

Drosophila, Human, Mouse, Non-Human Primate, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N241A/34 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N241A/34 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Leucine-rich repeat serine/threonine-protein kinase 2 (EC 2.7.11.1) (Dardarin)

UniProt ID

Q5S007

Shipping

Shipped on ice packs

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Anti-LRRK2/Dardarin, C-Terminus Recombinant Chicken Chimeric mAb (N241A/34)

SKU: 78-253

Product Details

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