GABA A receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR-beta2/3 a is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR beta2/3 has been found to be expressed in brain at the cell junction, synapse, postsynaptic cell membrane and cytoplasmic vesicle membrane.
EM, ICC, IHC, IP, WB
Fraction of bovine brain GABA-A receptor affinity purified against immobilized benzodiazepine Ro7-1986/1 (1988 de Blas et al J Neurosci PMID 2828565, 1988 Vitorica et al J Neurosci PMID 2828566). Target initially identified as a GABA-A receptor by: 1). rat brain immunocytochemistry staining pattern via light microscopy identical to that seen via ligand autoradiography with 3H-muscimol; 2). immunoprecipitation of 3H-muscimol-binding activity of the purified receptor complex; 3). recognition of ~57 kDa protein in immunoblots against crude rat brain membrane fractions Binding epitope identified to be within amino acids 26-40 (QSVNDPGNMSFVKET, extracellular N-terminus, 1992 Ewert et al Brain Res, PMID 1377081) of human GABA-A-R-Beta3 (also known as Gamma-aminobutyric acid receptor subunit beta-3, accession number P28472); Mouse: 100% identity (15/15 amino acids identical); Rat: 100% identity (15/15 amino acids identical); 86% identity (13/15 amino acids identical, QSVNDPSNMSLVKET) with GABA-A-R-Beta2; 66% identity (10/15 amino acids identical, HSTNEPSNMSYVKET) with GABA-A-R-Beta1; Hybridoma kindly donated by Angel de Blas, University of Connecticut. This NeuroMab antibody is considered "Restricted" and is therefore not available for commercial re-distribution on a for-profit basis.
Bovine, Chicken, Human, Mouse, Rat
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.