Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged Foxi3 or untagged Kv2.1 plasmid and probed with N359/28 TC supe (left), Mouse anti-Flag (middle) or N52A/42 TC supe (right).
Forkhead box I3
Fusion protein amino acids 1-127 (N-terminus) of mouse Foxi3 (also known as Forkhead box protein I3, RCG56131 and rCG_56131, accession number E0CZH3)
Rat: 94% identity (97/103 amino acids identical, with N-terminal 26 amino acid truncation)
Human: 73% identity (86/117 amino acids identical, with N-terminal 26 amino acid truncation)
Less than 50% identity with Foxi1 and Foxi2
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.