Detect endogenous levels of alpha-synuclein (SNCA), also known as PARK1, NACP, PARK4, by Western Blot, immunohistochemistry, and immunofluorescence using this knockout-validated mouse anti-alpha-synuclein antibody.
Sagittal section of formalin fixed, paraffin-embedded rat brain showing strong staining of alpha synuclein within the molecular layer of the cerebellum. Image at right shows higher magnification of indicated area of interest. Sections were stained with anti-alpha-synuclein (clone 6113-10) antibody at 1:5,000 dilution and detected with anti-mouse HRP.
Bulk Order Anti-Alpha-Synuclein Antibody
Alpha-synuclein (SNCA), also known as PARK1, NACP, PARK4, is a member of the synuclein family, which also includes beta- and gamma-synuclein. Alpha-synuclein is a highly conserved protein known to be abundant in neurons and especially presynaptic terminals where it serves multiple roles including regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Functionally, alpha-synuclein has been implicated in synaptic plasticity (Liu 2004) and the assembly of snare complexes (Burre 2010) and it is known to be associated with various neuropathologies including Parkinson's Disease, Lewy Body Dementia, and Alzheimer's Disease, where it forms insoluble protein aggregates. Early studies of amyloid deposits in the brains of Alzheimer's patients revealed two previously uncharacterized peptides in addition to the amyloid beta fragment (Ueda 1993), both of which were found to correspond to alpha-synuclein (SNCA). Later studies confirmed that aggregated alpha-synuclein proteins are present in brain lesions (Lewy bodies) that are hallmarks of neurodegenerative synucleinopathies and that alpha-synuclein likely plays a role in the pathogenesis of Parkinson's disease, Lewy body dementia, and Alzheimer’s disease among other neuropathologies. The SNCA antibody was raised against full-length human alpha-synuclein and recognizes endogenous levels of alpha-synuclein in brain by Western blot and immunohistochemistry.
Purified by Protein A Chromatography
ICC, IHC, WB
Full length human alpha-synuclein (accession number P37840) produced recombinantly in E. coli.
Human, Mouse, Non-Human Primate, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1 mg/mL.