Products: Primary Antibodies

Anti-Amyloid Precursor Protein (APP), Peptide #4


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PRODUCT OPTIONS

Catalog # Option Concentration Price*
Catalog #
APP4
Concentration
100 µg/ml
Price
$399.00

Overview

Brand:

Aves Labs

Antibody Type:

Polyclonal

Host Species:

Chicken

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC

Immunogen:

Chickens were immunized with a keyhole limpet hemocyanin (KLH) conjugate of the synthetic peptide EQK DRQ HTL KHF EHV RMV DPK K, which corresponds to residues #501-522 of the human gene product (NP_000475.1). This sequence is also conserved in the mouse and rat APP gene products, as well. After repeated injections, immune eggs were collected from the hens, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified against the cognate peptide using an agarose column, the concentration of the eluates adjusted to 100 µg/ml, and the preparation was filter-sterilized.

Protein Name(s):

Amyloid-beta A4 protein (ABPP) (APPI) (APP) (Alzheimer disease amyloid protein) (Amyloid precursor protein) (Amyloid-beta precursor protein) (Cerebral vascular amyloid peptide) (CVAP) (PreA4) (Protease nexin-II) (PN-II) [Cleaved into: N-APP; Soluble APP-alpha (S-APP-alpha); Soluble APP-beta (S-APP-beta); C99 (Beta-secretase C-terminal fragment) (Beta-CTF); Amyloid-beta protein 42 (Abeta42) (Beta-APP42); Amyloid-beta protein 40 (Abeta40) (Beta-APP40); C83 (Alpha-secretase C-terminal fragment) (Alpha-CTF); P3(42); P3(40); C80; Gamma-secretase C-terminal fragment 59 (Amyloid intracellular domain 59) (AICD-59) (AID(59)) (Gamma-CTF(59)); Gamma-secretase C-terminal fragment 57 (Amyloid intracellular domain 57) (AICD-57) (AID(57)) (Gamma-CTF(57)); Gamma-secretase C-terminal fragment 50 (Amyloid intracellular domain 50) (AICD-50) (AID(50)) (Gamma-CTF(50)); C31]

Gene Name(s):

APP A4 AD1


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
IHC Dilution Range:

1:1000-1:2000


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

This anti-peptide antibody mixture was analyzed by immunohistochemistry (at a dilution of 1:1000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.