Alkaline Phosphatase-Labeled Goat Anti-Chicken IgY Secondary Antibody

Name: Alkaline Phosphatase-Labeled Goat Anti-Chicken IgY Secondary Antibody
Brand: Aves Labs
SKU: AP-1001
Price: 140.0 USD
Availability: InStock

1 Citation

Our Alkaline Phosphatase-Labeled Goat Anti-Chicken IgY secondary antibody from Aves Labs is produced in-house. It detects chicken IgY and is affinity purified.

Species Reactivity

Chicken

Host Species

Goat

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Data Sheet

SKU: AP-1001

Volume: 1 mL

Variant

Price:

$140.00

Quantity:

Product Details

Target

Chicken IgY

Format

Affinity Purified

Concentration

0.1 mg/mL

Clonality

Polyclonal

Isotype

IgG

Host Species

Goat

Antigen

IgY

Species Reactivity

Chicken

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.

Physical State

Liquid

Production Notes

Goats were immunized with purified chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. Antibodies were isolated using affinity chromatography and conjugated to alkaline phosphatase.

Buffer

HEPES (50 mM, pH 7.0), NaCl (0.1 M), bovine serum albumin (0.2%, w/v), MgCl2 (1.0 mM), ZnCl2 (0.1 mM), sodium azide (0.02%, w/v) (as an antimicrobial agent).

Conjugate

Specificity

Does not cross-react with mammalian IgGs

Quality Control

Antibodies were analyzed in a sandwich ELISA format where a 1:800 dilution of GGHL-30PH-2 was found to generate an O.D of 1.0 in a 20 minute reaction with p-nitrophenyl phosphate as the substrate.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Shipping

Ice packs

Product Specific References for Applications

Western Blot

Western Blot
PMID	Dilution	Publication
18658160	not listed	Tollner, T.L., et al. 2008. Macaque sperm coating protein DEFB126 facilitates sperm penetration of cervical mucus. Human Reproduction, 2523-2534.

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Alkaline Phosphatase-Labeled Goat Anti-Chicken IgY Secondary Antibody

SKU: AP-1001

Product Details

Product Specific References for Applications

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