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Products: Primary Antibodies

Anti-Actin Antibody (Nitrocellulose Membrane Loading Control) Antibody

Aves Labs Logo from Aves Labs

 Datasheet

Product Summary:

Our anti-actin antibody (made against rabbit muscle actin) recognizes mouse, rat and human actin proteins in SDS-polyacrylamide gel transfers onto nitrocellulose membranes. This loading control assures that similar amounts of total proteins were added to each of the lanes in the gels. Note that this product does not work in transfers using PVDF membranes.

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
Aves Labs Logo
Catalog #
ACT-1010
Option
100 µL
Volume
100 µL
Concentration
10 mg/mL
Price
$125.00
Brand
Aves Labs Logo
Catalog #
ACT-1040
Option
400 ul
Volume
400 µL
Concentration
10 mg/mL
Price
$255.00

Validation Images


Product Details

Antibody Type:

Polyclonal

Host Species:

Chicken

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

WB

Immunogen:

Chickens were immunized with actin purified from rabbit muscle. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column. The final product contains 50% glycerol (v/v), purified anti­actin IgY fraction (10 mg/mL) spiked with 25 ug/ml of affinity purified anti-actin IgY, and then filter-sterilized.

Antibody Registry ID:

100 µL: AB_2313504
400 ul: AB_2313503


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Molecular Weight:

42 kDa

Western Dilution Range:

1:2500-1:5000


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

This anti-actin IgY preparation was analyzed by western blot analysis (at a dilution of 1:2000) using HRP-labeled goat anti-chicken IgY (1:5000 dilution, Aves Labs Cat.# H-1004) as the secondary reagent.


Citations and References