Products: Primary Antibodies

Anti-Actin Antibody (Nitrocellulose Membrane Loading Control)

from Aves Labs

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # ACT-1010	Option 100 µL	Volume 100 µL	Concentration 10.0 mg/ml	Price $125.00
Brand	Catalog # ACT-1040	Option 400 ul	Volume 400 ul	Concentration 10.0 mg/ml	Price $255.00

Validation Images

Overview

Brand:

Aves Labs

Antibody Type:

Polyclonal

Host Species:

Chicken

Species Reactivity:

Human Mouse Rat

Applications:

Immunogen:

Chickens were immunized with actin purified from rabbit muscle. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column. The final product contains 50% glycerol (v/v), purified antiactin IgY fraction (10 mg/ml) spiked with 25 ug/ml of affinity purified anti-actin IgY, and then filter-sterilized.

Antibody Registry ID:

100 µL: AB_2313504
400 ul: AB_2313503

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Molecular Weight:

42 kDa

Western Dilution Range:

1:2500-1:5000

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

This anti-actin IgY preparation was analyzed by western blot analysis (at a dilution of 1:2000) using HRP-labeled goat anti-chicken IgY (1:5000 dilution, Aves Labs Cat.# H-1004) as the secondary reagent.

Citations and References

Tian S, Muneeruddin K, Choi MY, Tao L, Bhuiyan RH, Ohmi Y, Furukawa K, Furukawa K, Boland S, Shaffer SA, Adam RM, Dong M. (2018), 'Genome-wide CRISPR screens for Shiga toxins and ricin reveal Golgi proteins critical for glycosylation.' Plos Biology. 10.1371/journal.pbio.2006951.