Products: Primary Antibodies
PRODUCT OPTIONS
| Catalog # | Option | Volume | Concentration | Price* | |
|---|---|---|---|---|---|
| Catalog # ACT-1010 |
Option 100 µL |
Volume 100 µL |
Concentration 10.0 mg/ml |
Price $115.00 |
|
| Catalog # ACT-1040 |
Option 400 ul |
Volume 400 ul |
Concentration 10.0 mg/ml |
Price $245.00 |
Validation Images
Overview
Aves Labs
Polyclonal
Chicken
Human Mouse Rat
WB
Chickens were immunized with actin purified from rabbit muscle. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column. The final product contains 50% glycerol (v/v), purified antiactin IgY fraction (10 mg/ml) spiked with 25 ug/ml of affinity purified anti-actin IgY, and then filter-sterilized.
100 µL: AB_2313504
400 ul: AB_2313503
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
42 kDa
1:2500-1:5000
Quality Control
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
This anti-actin IgY preparation was analyzed by western blot analysis (at a dilution of 1:2000) using HRP-labeled goat anti-chicken IgY (1:5000 dilution, Aves Labs Cat.# H-1004) as the secondary reagent.
Citations and References
- Tian S, Muneeruddin K, Choi MY, Tao L, Bhuiyan RH, Ohmi Y, Furukawa K, Furukawa K, Boland S, Shaffer SA, Adam RM, Dong M. (2018), 'Genome-wide CRISPR screens for Shiga toxins and ricin reveal Golgi proteins critical for glycosylation.' Plos Biology. 10.1371/journal.pbio.2006951.