Services: Antibody Development

Polyclonal Antibody Development

Custom antibody development is rarely a "cookie cutter" project.

Your project has price and technical requirements in addition to specific expectations for the performance characteristics of the antibodies produced. Our Antibody Development Group adds value by partnering with your team to evaluate these considerations with you and tailoring the project to meet your needs.  

We can help you select the optimal host animal.

Some host animal options include Chicken, Rabbit, Goat/Sheep and Alpaca. Additional hosts are available as well. Consult our host selection guide to learn more about the particular advantages and limitations of each host animal, and get in touch with us for further guidance. Or, if you already know which host animal you would like to use, see below for processes and pricing.

Process Overview by Host

Chicken Polyclonal Antibody Development

Immunogen Selection (Variable timeline)

  • Determination of optimal immunogen for your project.
  • We can either use an immunogen supplied by you or, in consultation with you, we can design and synthesize a peptide immunogen that meets your criteria (species reactivity, degree of reactivity with related proteins etc.).
     

    Immunization Schedule (10 weeks)

    • Purified Immune IgY from two (2) chickens (approx. 800 mg of IgY per hen)
    • Pre-Immune IgY from each chicken (~150mg of IgY per hen)
    • Four (4) Immunizations per animal.
       

      ELISA Report (Less than one week)

      • Eggs are collected and IgY is analyzed by ELISA and compared to the pre-immune eggs to evaluate titer (recommended).
         

        IgY Purification (1 week)

        • Twelve (12) eggs are used to prepare purified IgY using our proprietary protocol yielding an IgY preparation that is >90% pure.
        • The purified IgY prep can be used in most common applications (immunohistochemistry, immunocytochemistry, Western blotting etc.).
        • To obtain only antigen-specific binders, the IgY prep can be further subjected to affinity purification (see below).
           

          Affinity Purification (2 weeks)

          • The IgY fraction is subjected to antigen-specific affinity purification yielding an antigen-specific antibody preparation that is >90% pure.


            Pricing: Starts at $1,400

               

              Rabbit Polyclonal Antibody Development

              Immunogen Selection (Variable timeline)

              • Determination of optimal immunogen for your project.
              • We can either use an immunogen supplied by you or, in consultation with you, we can design and synthesize a peptide immunogen that meets your criteria (species reactivity, degree of reactivity with related proteins etc.).
                 

                Immunization and Bleeds (8 weeks)

                • Collection of 10mL pre-immune bleed for use as control (~6ml serum)
                • 57 day immunization protocol- includes 5 immunizations and a test bleed taken after 3rd immunization.
                • Single production bleed taken after 5th immunization (~15mL serum)
                • Serum can be used directly in most common assays (immunohistochemistry, immunocytochemistry, Western blotting etc.) or purified by either Protein A or affinity purification (see below).
                   

                  ELISA Report (Less than one week)

                  • Test bleed analyzed by ELISA and compared to the pre-immune bleed to evaluate titer (recommended).
                     

                    Protein A Purification (2 weeks)

                    • Production bleed is subjected to protein A purification yielding an IgG preparation that is >90% pure.
                    • The purified IgG prep can be used in most common applications (immunohistochemistry, immunocytochemistry, Western blotting etc.).
                    • To obtain only antigen-specific binders, the production bleed can alternatively be purified by affinity purification (below)
                       

                      Affinity Purification (2 weeks)

                      • Production bleed is subjected to antigen-specific affinity purification yielding an antigen-specific antibody preparation that is >90% pure.


                        Pricing: Starts at $1,400

                         

                        Goat/Sheep Polyclonal Antibody Development

                        Immunogen Selection (Variable timeline)

                        • Determination of optimal immunogen for your project.
                        • We can either use an immunogen supplied by you or, in consultation with you, we can design and synthesize a peptide immunogen that meets your criteria (species reactivity, degree of reactivity with related proteins etc.).
                           

                          Immunization and Bleeds (8 weeks)

                          • Collection of 10mL pre-immune bleed for use as control
                          • 81-day immunization protocol- includes two test bleeds taken after third and fifth immunizations.
                          • Single production bleed taken after 5th immunization (~250mL serum-based on weight of goat/sheep)
                          • Serum can be used directly in most common assays (immunohistochemistry, immunocytochemistry, Western blotting etc.) or purified by either Protein A or affinity purification (see below).
                             

                            ELISA Report (Less than one week)

                            • First and second test bleeds are analyzed by ELISA and compared to the pre-immune bleed to evaluate titer (recommended).
                               

                              Protein A Purification (2 weeks)

                              • Production bleed is subjected to protein A purification yielding an IgG preparation that is >90% pure.
                              • The purified IgG prep can be used in most common applications (immunohistochemistry, immunocytochemistry, Western blotting etc.).
                              • To obtain only antigen-specific binders, the production bleed can alternatively be purified by affinity purification (below)
                                 

                                Affinity Purification (2 weeks)

                                • Production bleed is subjected to antigen-specific affinity purification yielding an antigen-specific antibody preparation that is >90% pure.


                                  Pricing: Starts at $1,400

                                   

                                  Alpaca Polyclonal Antibody Development

                                  Immunogen Selection (Variable timeline)

                                  • Alpacas generally do not mount robust immune responses against small molecules or peptides.
                                  • They have gained popularity due to the relative ease of cloning intact "single-domain" antibodies from their immune repertoire for use in phage display screening. Customers typically submit recombinant protein antigens for use as immunogens.
                                     

                                    Immunization and Bleeds (8 weeks)

                                    • Collection of 10mL pre-immune bleed for use as control
                                    • 63-day immunization protocol includes two test bleeds taken after second and fourth immunizations.
                                    • Single production bleed taken after fifth immunization, up to ~250mL whole blood (can be shipped as whole blood or sent as serum).
                                       

                                      ELISA Report (Less than one week)

                                      • First and second test bleeds are analyzed by ELISA and compared to the pre-immune bleed to evaluate titer (recommended).
                                         

                                        Pricing: Starts at $1,900

                                         

                                        Ready to Get Started?

                                        To receive a consultation and quote from one of our scientists, submit your project details below. We will respond within two business days. If you require immediate assistance, call us at (800) 824-8540.
                                         

                                        Thanks for your inquiry. The information you submitted has been sent to our Antibody Services Group. Typically, the team reviews submitted requests for 1-2 business days before responding so that we may offer substantial guidance from our first contact. If you need an immediate response, feel free to contact us at 1 (800) 824-8540. We look forward to working with you!
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