Services

Antibody / Antigen Conjugation

Conjugation via common conjugates at various scales

To be useful as detection and capture reagents, antibodies often are conjugated to fluorophores, enzymes, biotin and other such molecules. Similarly, recombinant proteins often are conjugated to allow them to efficiently couple with solid supports, or to improve their immunogenicity for antibody development.

We provide conjugation services using many of the commonly used conjugates and at various scales.

Conjugate Overview

Biotin
(2-100mg)

  • Used to label both antibodies and recombinant proteins for the purposes of coupling to streptavidin-coated substrates.
  • Biotin-conjugates are useful for:
    • immobilization of biotinylated antibody/protein to solid-phase supports (assay plates, beads, etc.); and
    • labeling primary antibodies to allow recognition by Streptavidin conjugates for immunofluorescence, flow cytometry, Western blotting, etc.
       

FITC
(2-100mg)

  • Used to label antibodies for use in fluorescence microscopy, cell sorting, etc.
     

HRP
(2-100mg)

  • Used to label antibodies for the purpose of detection by Western blotting, ELISA, and Immunohistochemistry.
     

KLH (Key Limpet Hemocyanin)
(2-10mg)

  • KLH is the protein most commonly used for conjugation to immunogens.
  • Small molecular weight peptides, highly conserved and "self" proteins are generally non-immunogenic.
  • Conjugation to KLH renders them immunogenic for the purpose of antibody production.

 

Ready to Get Started?

To receive a consultation and quote from one of our scientists, submit your project details below. We will respond within two business days. If you require immediate assistance, call us at (800) 824-8540.
 

Thanks for your inquiry. The information you submitted has been sent to our Antibody Services Group. Typically, the team reviews submitted requests for 1-2 business days before responding so that we may offer substantial guidance from our first contact. If you need an immediate response, feel free to contact us at 1 (800) 824-8540. We look forward to working with you!
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