Aves Labs

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Chicken anti-SNCA (green) and TH (red) double immunostaining in Parkinson's Disease patient’s substantia nigra section. Arrows indicate SNCA positive, degenerating dopamine neurons. Arrowheads indicate SNCA negative, healthy dopamine neurons. Bar = 50 um for A-D. (Image courtesy of Dr. Curt Freed’s lab, University of Colorado Anschutz Medical Campus.)Rat brain lysate was probed with no primary antibody as a control (Lane 1) or with a 1:2,500 dilution of Chicken anti-SNCA IgY (Lane 2).
Beta-Amyloid-positive “neuritic plaque” (shown in higher magnification) in cerebral cortex as seen in a post-mortum specimen taken from an Alzheimer’s disease patient. Picture courtesy of Dr. Randy Woltjer, Oregon Health & Sciences University.Immunohistochemistry of a capillary showing beta-amyloid peptide in the wall of the vessel. Paraffin-embedded section of a human brain from a patient with diagnosed Alzheimer's Disease. Primary antibody -- 1:500; secondary antibody -- 1:1000 HRP-Goat anti-chicken IgY (Aves Labs). Dr. Randy Woltjer, Dept Pathology, OHSU
Western blot. Left Lane -- Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. Lane 1, 30 µg protein; lane 2, 20 µg protein; lane 3, 10 µg protein; lane 4, 5 µg protein.
Co-staining of Coronin1 (COR, green, 1:1000 dilution) and Internexin (rabbit antibody, red, 1:500) in a culture of mouse cerebral cortical tissue fixed with 4% paraformaldehyde. Photo by Dr. Gerry Shaw, Univ. Florida.Co-staining of Coronin1 (COR, green, 1:1000 dilution) and Internexin (rabbit antibody, red, 1:500) in a culture of mouse cerebral cortical tissue fixed with 4% paraformaldehyde. Photo by Dr. Gerry Shaw, Univ. Florida.
Western blot. Right Lane -- Cultured HEK cells were transfected with a plasmid containing the RDF1 cDNA fused with a FLAG epitope tag at its C-terminus. Left Lane -- Cultured HEK cells with an empty plasmid vector. 25 μg of protein loaded in each lane.
Glial Fibrillary Acidic Protein (GFAP) immunostaining (red) of mixed cortical mouse brain cultures (1:500 dilution). Secondary antibody is Texas Red goat anti-chicken IgY. The blue is DAPI staining of nuclei of all cells, including non-GFAP-positive cells. Photo courtesy of Dr. Gerry Shaw, Univ. Florida.Immunohistochemical staining of GFAP-positive radial glial cells in the periventricular zone of an e16 mouse brain. Section was a vibratome, thick section (20 um) using a lightly-fixed (2% paraformaldehyde) mouse brain. 1˚ antibody (GFAP) was used at 1:500 dilution; 2˚ antibody (HRP-goat anti-chicken IgY) was used at 1:500 dilution.
Mixed neuron-glial cultures stained with CPCA-GAP43 (green) and rabbit antibody to alpha-II spectrin (RPCA-aII-Spec, red), and DNA (blue). The GAP43 antibody stains numerous axonal and dendritic profiles in these cultures, clearly revealing the submembraneous cytoskeleton and vesicles. Photo by Dr. Gerry Shaw, Univ. Florida.GAP-43 immunoreactivity in a neuroblast within a neurosphere culture. Chicken anti-GAP-43 antibody (1:500 dilution), Fluorescein-goat anti-chicken IgY secondary antibody (Aves Labs, 1:500). Photomicrograph by Hoda Ilias, Aves Labs.
Western blot. Left Lane -- Cultured COS cells were transfected with a plasmid containing the SAP1a cDNA fused with an HA epitope tag at its C-terminus. Right Lane -- COS transfected with an empty plasmid vector. 25 µg of protein loaded in each lane.Immunolabeling of mouse hindbrain dorsal motor nucleus of the vagus (DMV) neurons expressing an hM3Dq-HA tag (Anti-HA Epitope Tag, Cat ET-HA100, 1:200). The image is kindly provided by Nicholas Conley, Neuroscience Graduate Program, University of Virginia.
Immunofluorescence of AIF1/IBA1-FLAG transfected COS-7 cells using chicken α-AIF1/IBA1 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.Western blot of rat brain membrane lysate using chicken α-AIF1/IBA1 showing specific immunolabeling of endogenous AIF1/IBA1 at ~17kDa.
Aves Labs Anti-Iba1/AIF1 Antibody
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