Products: Primary Antibodies

Anti-PICK1 Antibody (L20/8)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-040	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $285.00
Brand	Catalog # 73-040	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $285.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP WB

Target Description:

PRKCA-binding protein or PICK1 scaffold is encoded by the gene PICK1. PICK1 is a scaffold protein which acts as an adaptor protein that binds and organizes the location of membrane proteins. Functions to cluster receptors and plays a role in synaptic plasticity. PICK1 is ubiguitously expressed and found in high levels in brain and testis. Cellularly, PICK1 is found at perinuclear region, mitochondria and synapses. Diseases associated with PICK1 include Spermatogenic Failure and Depression.

Immunogen:

Fusion protein amino acids 10-130 of rat PICK1 (accession number AAG48152); Human: 100% identity (121/121 amino acids); Mouse=99% identity (120/121 amino acids)

Cross Reactivity:

No cross-reactivity reported

Protein Name(s):

PICK1

Gene Name(s):

PICK1 PRKCABP

Antibody Registry ID:

Purified: AB_2164544
TC Supernatant: AB_10672986

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

45 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Back to Primary Antibodies