Products: Primary Antibodies

Anti-Nav1.2 Na+ channel Antibody (K69/3)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-024	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $285.00
Brand	Catalog # 73-024	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $285.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

$Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with K69/3 (left) or N52A/42 (right) TC supe.$

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP WB

Target Description:

Nav1.2 Na+ channel (sodium channel, voltage-gated, type II, alpha subunit/ SCN2A) is a member of voltage-gated sodium ion channel subunit family. It is encoded by gene Scn2a in human.The channel switches between open and close conformation in response to the voltage difference accross the membrane. Nav1.1 Na+ channel is a sodium selective channel that maintains Na+ homeostasis by allowing Na+ ions to pass in accordance of their electrochemical gradient.Nav1.2 Na+ channel is implicated in the regulation of hippocampal replay, whose waves ripples (SPW-R) condition memories. It is heterogeneously expressed in the brain, and mutations in this gene have been linked to several cases of seizures, autism and bipolar disorders.

Immunogen:

Fusion protein amino acids 1882-2005 (cytoplasmic C-terminus) of rat rat Nav1.2 (accession number P04775); Human: 94% identity (117/124); Mouse: 100% identity (124/124)

Cross Reactivity:

Lack of cross-reactivity against Nav1.1, Nav1.3, Nav1.6 verified

Protein Name(s):

Nav1.2 Na+ channel

Gene Name(s):

Scn2a Scn2a1

Antibody Registry ID:

Purified: AB_2184030
TC Supernatant: AB_10673401

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

˜250 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

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