Products: Primary Antibodies

Anti-GluN2B/NR2B glutamate receptor Antibody (N59/36)

from NeuroMab

PRODUCT OPTIONS

★ Trial Size Available ★

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-101	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $335.00
Brand	Catalog # 75-101-020	Option Purified Trial-Size	Volume 20 µL	Concentration 1 mg/mL	Price $119.00
Brand	Catalog # 73-101	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $335.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP WB

Target Description:

GluN2B/NR2b glutamate receptor, also known as Glutamate/N-methyl D-aspartate/NMDA receptor subtype 2B, is a member of the NMDA receptor family. NMDA receptors are expressed throughout the CNS in postsynaptic cell membranes. GluN2B is a major NMDAR subtype found in cortical and hippocampal regions of the adult brain. NMDA receptors are involved with memory and learning. Mutations in the gene encoding GluN2B are associated with autism, LennoxGastaut and West Syndromes.

Immunogen:

Fusion protein amino acids 20-271 (extracellular N-terminus) of rat NR2B (also known as N-methyl D-aspartate/NMDA receptor subtype 2B or accession number Q00960); Human: 99% identity (250/252 amino acids identical); Mouse: 99% identity (250/252 amino acids identical)

Cross Reactivity:

Does not cross-react with GluN2A/NR2A

Protein Name(s):

GluN2B/NR2B glutamate receptor

Gene Name(s):

Grin2b

Antibody Registry ID:

Purified: AB_2232584
Purified Trial-Size: AB_2232584
TC Supernatant: AB_10672980

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

166 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

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