Human Mouse Rat
Glutamate receptor NMDA2A or GluN2A/NR2A glutamate receptor is a member of the glutamate-gated ion channel family. NR2A is a subunit of the ligand-gated ion channel with high calcium permeability and voltage-dependent sensitivity to magnesium. NR2A is expressed throughout the brain, including striatum, forbrain, cerebellum (at protein level) brain cortex, piriform cortex, hippocampus, caudate-putamen, dentate gyrus and granule cell layer. It is found at the postsynaptic cell membrane, dendritic spine, synapse, and cytoplasmic vesicle membrane. A variety of neurological diseases, including epilepsy, intellectual disability, autism spectrum disorders, developmental delay, and schizophrenia are associated with mutations in the NR2A gene.
Fusion protein amino acids 75-325 (extracellular N-terminus) of rat NR2A (also known as Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D aspartate receptor subtype 2A, Grin2a, NMDAR2A, accession number Q00959)
Mouse: 99% identity (250/251 amino acids identical)
Human: 97% identity (245/252 amino acids identical)
>60% identity with NR2B
Does not cross-react with GluN2B/NR2B
Glutamate receptor ionotropic, NMDA 2A (GluN2A) (Glutamate [NMDA] receptor subunit epsilon-1) (N-methyl D-aspartate receptor subtype 2A) (NMDAR2A) (NR2A)
TC Supernatant: AB_2315841
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
Citations and References
- Moraga-Amaro R1, González H2, Ugalde V2, Donoso-Ramos JP1, Quintana-Donoso D1, Lara M3, Morales B3, Rojas P3, Pacheco R4, Stehberg J5.. (2016), 'Dopamine receptor D5 deficiency results in a selective reduction of hippocampal NMDA receptor subunit NR2B expression and impaired memory..' Neuropharmacology.. 10.1016/j.neuropharm.2015.12.018.
- Berg JM1, Lee C2, Chen L2, Galvan L3, Cepeda C3, Chen JY3, Peñagarikano O2, Stein JL2, Li A2, Oguro-Ando A2, Miller JA2, Vashisht AA4, Starks ME2, Kite EP2, Tam E2, Gdalyahu A5, Al-Sharif NB3, Burkett ZD6, White SA6, Fears SC3, Levine MS3, Wohlschlegel JA4, Geschwind DH7. (2015), 'JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse..' Neuron. . 10.1016/j.neuron.2015.10.031.
- Balsara R1, Dang A2, Donahue DL2, Snow T1, Castellino FJ1.. (2015), 'Conantokin-G attenuates detrimental effects of NMDAR hyperactivity in an ischemic rat model of stroke..' PLoS One.. 10.1371/journal.pone.0122840.
- Segovia-Miranda F1, Serrano F1, Dyrda A2, Ampuero E2, Retamal C1, Bravo-Zehnder M1, Parodi J3, Zamorano P1, Valenzuela D4, Massardo L1, van Zundert B2, Inestrosa NC5, González A5.. (2015), 'Pathogenicity of lupus anti-ribosomal P antibodies: role of cross-reacting neuronal surface P antigen in glutamatergic transmission and plasticity in a mouse model..' Arthritis Rheumatol.. 10.1002/art.39081.
- D'Orsi B1, Kilbride SM1, Chen G1, Perez Alvarez S1, Bonner HP1, Pfeiffer S1, Plesnila N2, Engel T1, Henshall DC1, Düssmann H1, Prehn JH3.. (2015), 'Bax regulates neuronal Ca2+ homeostasis..' J Neurosci.. 10.1523/JNEUROSCI.2453-14.2015.
- Bhattacharyya M, Nandanoor A, Osman M, Kasinathan C, Frederikse P.. (2014), 'NMDA glutamate receptor NR1, NR2A and NR2B expression and NR2B Tyr-1472 phosphorylation in the lens..' Neurochem Res.. 10.1007/s11064-014-1394-z.
- Ricciardi S1, Ungaro F, Hambrock M, Rademacher N, Stefanelli G, Brambilla D, Sessa A, Magagnotti C, Bachi A, Giarda E, Verpelli C, Kilstrup-Nielsen C, Sala C, Kalscheuer VM, Broccoli V.. (2012), 'CDKL5 ensures excitatory synapse stability by reinforcing NGL-1-PSD95 interaction in the postsynaptic compartment and is impaired in patient iPSC-derived neurons..' Nat Cell Biol.. 10.1038/ncb2566.