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Products: Primary Antibodies

Anti-GluN2A/NR2A Antibody (N327/95)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-288
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$335.00
Brand
NeuroMab Logo
Catalog #
73-288
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$335.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

IHC WB

Target Description:

Glutamate receptor NMDA2A or GluN2A/NR2A glutamate receptor is a member of the glutamate-gated ion channel family. NR2A is a subunit of the ligand-gated ion channel with high calcium permeability and voltage-dependent sensitivity to magnesium. NR2A is expressed throughout the brain, including striatum, forbrain, cerebellum (at protein level) brain cortex, piriform cortex, hippocampus, caudate-putamen, dentate gyrus and granule cell layer. It is found at the postsynaptic cell membrane, dendritic spine, synapse, and cytoplasmic vesicle membrane. A variety of neurological diseases, including epilepsy, intellectual disability, autism spectrum disorders, developmental delay, and schizophrenia are associated with mutations in the NR2A gene.

Immunogen:

Fusion protein amino acids 75-325 (extracellular N-terminus) of rat NR2A (also known as Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D aspartate receptor subtype 2A, Grin2a, NMDAR2A, accession number Q00959) Mouse: 99% identity (250/251 amino acids identical)
Human: 97% identity (245/252 amino acids identical)
>60% identity with NR2B

Cross Reactivity:

Does not cross-react with GluN2B/NR2B

Protein Name(s):

Glutamate receptor ionotropic, NMDA 2A (GluN2A) (Glutamate [NMDA] receptor subunit epsilon-1) (N-methyl D-aspartate receptor subtype 2A) (NMDAR2A) (NR2A)

Gene Name(s):

Grin2a

Antibody Registry ID:

Purified: AB_2315842
TC Supernatant: AB_2315841


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

170 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.


Citations and References