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Products: Primary Antibodies

Anti-GFAP Antibody (N206A/8)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

★ Trial Size Available ★
Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-240
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$335.00
Brand
NeuroMab Logo
Catalog #
75-240-020
Option
Purified Trial-Size
Volume
20 µL
Concentration
1 mg/mL
Price
$119.00
Brand
NeuroMab Logo
Catalog #
73-240
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$335.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Target Description:

Glial fibrillary acidic protein (GFAP) is an intermediate filament with many different isoforms, and acts as a structural protein in the cytoskeleton. It is expressed in astrocytes in brain and is often used as an astrocyte marker. GFAP is belived to be involved in many CNS processes including cell communication and mitosis. GFAP has been found to be involved in Alexander disease and Wernicke's encephalopathy.

Immunogen:

Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP (also known as Glial fibrillary acidic protein, accession number P14136); Rat: 100% identity (12/12 amino acids identical); Mouse: 100% identity (12/12 amino acids identical)
Greater than 50% identity with other proteins (Vimentin, Desmin, Tumor protein p73 and Peripherin)

Cross Reactivity:

Cross-reacts with GFAP-R416W and other GFAP mutant proteinsDoes not cross-react with other proteins (based on KO validation results)

Protein Name(s):

GFAP

Gene Name(s):

GFAP

Antibody Registry ID:

Purified: AB_10672299
Purified Trial-Size: AB_10672299
TC Supernatant: AB_10672298


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

50 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.