Products: Primary Antibodies

Anti-GABA(A)R, Beta3 Antibody (N87/25)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-149	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $285.00
Brand	Catalog # 73-149	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $285.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

$Immunoblot against membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N87/25 (left) or N52A/42 (right) TC supe.$

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Target Description:

Gamma-aminobutyric acid receptor subunit beta-3 or GABA AR-Beta3 is encoded by the gene GABRB3. GABAA receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR-beta3 a is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR beta3 has been found to be expressed in retina, heart and primarily in brain at the cell junction, synapse, postsynaptic cell membrane and cytoplasmic vesicle membrane. This gene may be associated with the pathogenesis of several disorders including epilepsy and autism, childhood absence epilepsy 5 and early infantile epileptic encephalopathy 43.

Immunogen:

Fusion protein amino acids 370-433 of mouse GABA-A-R-Beta3 (also known as gamma-aminobutyric acid receptor subunit beta-3, accession number AAB60502); Rat: 100% identity (64/64 amino acids identical); Human: 85% identity (53/64 amino acids identical); Less than 40% identity with GABA-A-R-Beta1 or -Beta2

Cross Reactivity:

No cross-reactivity against GABA-A-R-Beta1 or –Beta2

Protein Name(s):

GABA(A)R, Beta3

Gene Name(s):

Gabrb3 Gabrb-3

Antibody Registry ID:

Purified: AB_2109585
TC Supernatant: AB_10673389

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

55 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

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