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Products: Primary Antibodies

Anti-Arl13b Antibody (N295B/66)

NeuroMab Logo from NeuroMab

 Datasheet

Product Summary:

Detect Arl13b with this antibody validated for IHC, WB, ICC.

PRODUCT OPTIONS

★ Trial Size Available ★
Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-287
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$335.00
Brand
NeuroMab Logo
Catalog #
75-287-020
Option
Purified Trial-Size
Volume
20 µL
Concentration
1 mg/mL
Price
$119.00
Brand
NeuroMab Logo
Catalog #
73-287
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$335.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Target Description:

ADP-ribosylation factor-like protein 13B (ARL13B), also known as ADP-ribosylation factor-like protein 2-like 1. Arl13B is a small GTPase with both N-terminal and C-terminal guanine nucleotide-binding motifs. Arl13B is localized to the membrane of cilia and expressed in Neuroepithelial cells and developing radial glia of the developing cerebral cortex. Mutations in this gene are the cause of Joubert syndrome 8.

Immunogen:

Fusion protein amino acids 208-427 (C-terminus) of mouse Arl13b (also known as ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1, ARL2-like protein 1 and Arl2l1, accession number Q640N2)
Rat: 85% identity (189/220 amino acids identical)
Human: 68% identity (154/224 amino acids identical)
<40% identity with Arl13a

Cross Reactivity:

Does not cross-react with Arl13a (based on KO validation results)

Protein Name(s):

ADP-ribosylation factor-like protein 13B (ADP-ribosylation factor-like protein 2-like 1) (ARL2-like protein 1) (Protein hennin)

Gene Name(s):

Arl13b Arl2l1

Antibody Registry ID:

Purified: AB_11001586
Purified Trial-Size: AB_11001586
TC Supernatant: AB_11000053


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

60 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.


Citations and References