Anti-Olig2 Antibody

Name: Anti-Olig2 Antibody
Brand: Aves Labs
SKU: OLIG2-0100
Price: 440.0 USD
Availability: InStock

from Aves Labs Datasheet

Trial Size

Detect endogenous levels of OLIG2 (also known as BHLHB1, BHLHE19, PRKCBP2, RACK17) by Western Blot, immunohistochemistry, and immunofluorescence using this highly-validated chicken anti-OLIG2 antibody.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

Format

Affinity Purified IgY

Gene Name

OLIG2

Molecular Weight

35 kDa

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Immunofluoresence of COS-7 cells expressing OLIG2-flag using AVES chicken anti-OLIG2 antibody (green) and rabbit anti-flag antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-OLIG2 signal and anti-flag in transfected cells.

Sagittal section of formalin fixed, paraffin-embedded rat brain showing nuclear staining of OLIG2 positive cells within the white matter of cerebellum as expected. Inset (top left) shows higher magnification. Sections were stained with AvesLabs chicken anti-OLIG2 antibody at 1:1,000 dilution and detected with anti-chicken HRP.

COS-7 cells were transfected with OLIG2-flag cDNA or mock transfected for 48 hours and lysed. Equal amounts of cell lysates were separated by SDS-PAGE and immunoblotted with chicken anti-OLIG2. AvesLabs Chicken anti-OLIG2 antibody recognizes OLIG2 protein but does not show any reactivity in the mock transfected COS-7 lysate.

Immunofluoresence of COS-7 cells expressing OLIG2-flag using AVES chicken anti-OLIG2 antibody (green) and rabbit anti-flag antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-OLIG2 signal and anti-flag in transfected cells.

Size: 500 µL

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$440

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Product Details

Target

Olig2

Target Description

OLIG2 (also known as BHLHB1, BHLHE19, PRKCBP2, RACK17) is a basic helix-loop-helix (bHLH) transcription factor essential for oligodendrocyte and motoneuron development in the spinal cord (Ono et al., 2008). OLIG2 is also known to play a key role in mediating the brains response to cortical injury through post-injury gliosis (Chen Y et al., 2008) and is also highly expressed in malignant glioma where it has been implicated in glioma progression.

Format

Affinity Purified IgY

Concentration

0.2 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

OLIG2

Molecular Weight

35 kDa

Antigen

N-terminal half of human OLIG2 was used to immunize chickens to avoid cross-reactivity with OLIG3.

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2924438

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.

Buffer

Phosphate-buffered saline (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:1000
IHC: 1:1000
ICC: 1:1000

Specificity

No cross reactivity reported.

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot on lysate from cells expressing Olig2.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

1 year from date of receipt

Shipping

Ice packs

Synonyms

BHLHB1, BHLHE19, PRKCBP2, RACK17

UniProt Details

UniProt (Human): Q13516
UniProt (Immunogen Species): Q13516

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Anti-Olig2 Antibody

Detect endogenous levels of OLIG2 (also known as BHLHB1, BHLHE19, PRKCBP2, RACK17) by Western Blot, immunohistochemistry, and immunofluorescence using this highly-validated chicken anti-OLIG2 antibody.