Anti-EphA4 (N-terminal region) Antibody (M280)

Name: Anti-EphA4 (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: EM2801
Price: 340.0 USD
Availability: InStock

3 Citations

Our EphA4 (N-terminal region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat EphA4 (N-terminal region) and is Protein A purified. It is great for use in WB.

Western blot analysis of human umbilical vein endothelial cells untreated (lanes 1, 3, 5, & 7) or treated with pervanadate (1 mM) for 30 min. (lanes 2, 4, 6, & 8). The blot was probed with anti-EphA4 (N-terminal region) (lanes 1 & 2), anti-EphA4 (Tyr-779) (lanes 3 & 4), anti-EphA4 (Tyr-602) (lanes 5 & 6), or anti-EphA4 (C-terminal region) (lanes 7 & 8).

Western blot image of various mouse and human HCC cell lines expressing EphA4 (Cat. EM2801, 1:1000). Image from publication CC-BY-4.0. PMID: 38307859

Western blot image of mouse HCA-1 (left) and human Hep3B (right) cells treated with RNase1 (1 ug/ml) at the indicated time points specifically labeling EphA4 (EM2801, 1:1000) and phosphorylated EphA4-Tyr604 (EP2731, 1:1000) Image from publication CC-BY-4.0. PMID: 38307859

Western blot analysis of human umbilical vein endothelial cells untreated (lanes 1, 3, 5, & 7) or treated with pervanadate (1 mM) for 30 min. (lanes 2, 4, 6, & 8). The blot was probed with anti-EphA4 (N-terminal region) (lanes 1 & 2), anti-EphA4 (Tyr-779) (lanes 3 & 4), anti-EphA4 (Tyr-602) (lanes 5 & 6), or anti-EphA4 (C-terminal region) (lanes 7 & 8).

Click on image to zoom

Species Reactivity

Chicken, Human, Mouse, Rat

Applications

Host Species

Mouse

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Data Sheet

SKU: EM2801

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Ships: 1-2 business days

Product Details

Target

EphA4 (N-terminal region)

Scientific Background

The Eph family of Receptor tyrosine kinases and their Ephrin ligands are important for cell positioning and morphogenesis during development. Eph receptors are classified into 10 EphA and 6 EphB receptors, which preferentially bind to the type A and type B ephrins, respectively. The EphA4 receptor can inhibit axon outgrowth and has roles in regulating axon projections during neural development. EphA4 signaling pathways require its kinase activity and involve binding and activation of Rho-GTPase guanine nucleotide-exchange factors (GEFs). EphA4 activation leads autophosphorylation of Tyr-596 and Tyr-602, and the conserved sites in EphA2 are required for binding to the GEFs, Vav2 and Vav3, and ephrin-induced cell migration. The Tyr-779 site in the kinase domain is also phosphorylated in vivo and may regulate kinase activity. Activated EphA4 leads to Src kinase phosphorylation of the GEF, ephexin-1, and this activates RhoA. Thus, EphA4 signaling involves complex tyrosine phosphorylation in its cytoplasmic region along with interaction with several GEFs.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M280

Isotype

IgG1

Applications

Host Species

Mouse

Gene Name

EPHA4

Molecular Weight

95/120

Immunogen

Clone M280 was generated from a recombinant protein corresponding to amino acids in the N-terminal region of human EphA4. This region has less than 50% homology to similar regions in other EphA and EphB family members.

Immunogen Species

Human

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

This antibody detects 95 and 120 kDa* bands corresponding to EphA4 in Western blots of HUVEC cells and a 120 kDa band in mouse brain tissue.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

SEK, Eph

UniProt ID

P54764

Shipping

Blue Ice

Product Specific References for Applications and Species

Western Blot: Human | Mouse

Western Blot: Human
PMID	Dilution	Publication
38307859	1:1000	Liu, C, et al. 2024. Targeting ALK averts ribonuclease 1-induced immunosuppression and enhances antitumor immunity in hepatocellular carcinoma. Nature communications, 1009.

Western Blot: mouse
PMID	Dilution	Publication
38307859	1:1000	Liu, C, et al. 2024. Targeting ALK averts ribonuclease 1-induced immunosuppression and enhances antitumor immunity in hepatocellular carcinoma. Nature communications, 1009.
29789418	1:1000	Yang, WH, et al. 2018. Juxtacrine Signaling Inhibits Antitumor Immunity by Upregulating PD-L1 Expression. Cancer research, 3761-3768.
28526745	1:1000	Schoonaert, L, et al. 2017. Identification and characterization of Nanobodies targeting the EphA4 receptor. The Journal of biological chemistry, 11452-11465.

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Anti-EphA4 (N-terminal region) Antibody (M280)

SKU: EM2801

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-EphA4 (N-terminal region) Antibody

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