Answer:
Polyclonal Antibodies listed, with the exception of products #48-xxxx series, are not affinity purified and are excellent for procedures and techniques that do not require high specificity. They are prepared by immunizing host animals with purified IgG antigen preparations and represent a low cost alternative to affinity purified antibodies. These products can be used for applications such as: capture procedures for RIA and EIA; preparation of immunoabsorbents; immunoprecipitation; bridging antibodies for peroxidase-anti-proxidase (PAP) procedures; immunoelectrophoresis; and agglutination assays.

Answer:
Unlabeled antisera produced in goats and sheep have an average Ouchterlony preciptin titer of 8.0 mg/ml specific antibody. Antisera produced in rabbits have a minimum of 7.0 mg/ml. Store at zero to -20 degrees C.

Answer:
Fluorescein Isothiocynate (FITC) labeled antibodies have an average of 3.2 ug FITC per milligram of protein, supplied at 10 mg/ml for goats, sheep and rabbits. Store at zero to -20 degrees C. 
 
Horseradish Peroxidase (HRP) labeled antibodies are provided at 1.0 mg/ml in 0.01 M Phosphate Buffered Saline (PBS), pH 7.6 containing 10% mannose, 2% sucrose and 1% BSA as stabilizing reagents and 0.1% sodium azide as a preservative. Suggested working dilution is approximately 1:10,000 for direct ELISA. Store at 4 degrees C. 
 
Alkaline Phosphatase (AP) labeled antibodies are supplied at 1.0 mg/ml in 0.05 M TRIS-HCl, pH approximately 1:5,000 for direct ELISA. Store at 4 degrees C. 
 
Biotin labeled antibodies are supplied at 0.5 mg/ml in PBS, pH 7.6 with 1.0% BSA for stabilization and 0.1% sodium azide as a preservative. Suggested working dilution is 1:20,000 for direct ELISA. Store at zero to -20 degrees C.

Answer:
AFP: Affinity Purified 
AP: Alkaline Phosphatase 
BIO: Biotin 
SA: Salted Out Antisera 
FITC: Fluorescein Isothiocyanate 
HRP: Horseradish Peroxidase 
UN: Unlabeled

Answer:
Please contact us by clicking on Contact Us link on the above to request a Design Input Form sent to you. Please provide us with either a fax or physical mailing address where we can send you one. 
 
We have Design Input Forms for Custom Antibodies and ELISA Development. Please specify which form you'd like.