Question:
What are the concentrations of Labeled Antibodies?
Answer:
Fluorescein Isothiocynate (FITC) labeled antibodies have an average of 3.2 ug FITC per milligram of protein, supplied at 10 mg/ml for goats, sheep and rabbits. Store at zero to -20 degrees C. Horseradish Peroxidase (HRP) labeled antibodies are provided at 1.0 mg/ml in 0.01 M Phosphate Buffered Saline (PBS), pH 7.6 containing 10% mannose, 2% sucrose and 1% BSA as stabilizing reagents and 0.1% sodium azide as a preservative. Suggested working dilution is approximately 1:10,000 for direct ELISA. Store at 4 degrees C. Alkaline Phosphatase (AP) labeled antibodies are supplied at 1.0 mg/ml in 0.05 M TRIS-HCl, pH approximately 1:5,000 for direct ELISA. Store at 4 degrees C. Biotin labeled antibodies are supplied at 0.5 mg/ml in PBS, pH 7.6 with 1.0% BSA for stabilization and 0.1% sodium azide as a preservative. Suggested working dilution is 1:20,000 for direct ELISA. Store at zero to -20 degrees C.
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