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Frequently Asked Questions


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What working concentrations/dilutions do you recommend?
What is the concentration of the purified antibody?
Is the peptide or protein antigen available?
What is an MTA and why is one needed?
What is the protocol for immunofluorescence staining of cultured cells?
What is the protocol for preparing mammalian brain fractions?
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Are all Polyclonal Antibodies Affinity Purified?
What is the concentration of Polyclonal Antibodies?
What are the concentrations of Labeled Antibodies?
What do the abbreviations AFP, AP, BIO, SA, FITC, HRP, and UN represent?
Where can I find design input forms?
 
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Active Question
Technical Topics
Question:
What are the concentrations of Labeled Antibodies?

Answer:
Fluorescein Isothiocynate (FITC) labeled antibodies have an average of 3.2 ug FITC per milligram of protein, supplied at 10 mg/ml for goats, sheep and rabbits. Store at zero to -20 degrees C. 
 
Horseradish Peroxidase (HRP) labeled antibodies are provided at 1.0 mg/ml in 0.01 M Phosphate Buffered Saline (PBS), pH 7.6 containing 10% mannose, 2% sucrose and 1% BSA as stabilizing reagents and 0.1% sodium azide as a preservative. Suggested working dilution is approximately 1:10,000 for direct ELISA. Store at 4 degrees C. 
 
Alkaline Phosphatase (AP) labeled antibodies are supplied at 1.0 mg/ml in 0.05 M TRIS-HCl, pH approximately 1:5,000 for direct ELISA. Store at 4 degrees C. 
 
Biotin labeled antibodies are supplied at 0.5 mg/ml in PBS, pH 7.6 with 1.0% BSA for stabilization and 0.1% sodium azide as a preservative. Suggested working dilution is 1:20,000 for direct ELISA. Store at zero to -20 degrees C.

 

Antibodies Incorporated, P.O. Box 1560, Davis, California 95617-1560
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