Custom Polyclonal Antibodies

Your study can be improved by teaming with our antibody production experts. We are a USDA registered and NIH assured facility with over 40 years of antibody production experience. Our Institutional Animal Care and Use Committee inspects the animal facilities at least every six months, and evaluates each research protocol prior to project commencement. We will tailor protocols to your specific needs, modified from the recommended standard 91-day protocol for large animal polyclonal antibody production, a standard 57-day protocol for rabbit, fowl, and large rodent polyclonal antibody production, and a standard 45-day protocol for mouse polyclonal antibody production. To place your Polyclonal or Monoclonal Antibody Production order, call Antibodies Incorporated today. 
 

DAY	0	1	14	21	28	35	49	57
Procedure	Pre-Bleed	1st Injection	2nd Injection	3rd Injection	Test Bleed	4th Injection	5th Injection	Production Bleed

 
After the production bleed (at 57 days) and an evaluation of the antisera, one of the following options can be chosen: 
 
Option A 
Antisera is acceptable. 
End the program. There is no additional cost for this option. 
 
Option B 
Bleed for maximum volume. For maximizing the titer, we recommend one more injection and a final bleed 8 days post injection. The cost for this option varies per species. For rabbits, the cost is $2.25/rabbit/day care and maintenance, $11.25/rabbit/injection, and $3.50/mL antiserum collected. For guinea pigs, the cost is $1.25/guinea pig/day care and maintenance, $11.25/guinea pig/injection, and $60.00/mL antiserum collected. For other species, please inquire. 
 
Option C 
Production. Run a production program with one injection each month and a production bleed 8 days later. The cost for this option varies per species. For rabbits and guinea pigs, the cost is the same as is listed in option B. For other species, please inquire. 
 
Antigen 
As least 10 µg of antigen / injection may be required for your antisera production. (We recommend 100 and 500 µg of antigen / injection.) Pathogenic, toxic, or carcinogenic antigens will not be accepted. 
 
Adjuvants 
Our standard adjuvant is Freunds (complete for 1st injection / incomplete for subsequent injections); however, we can customize the protocol and accommodate other commercially available adjuvants, upon request. 
 
Assay 
We can assay your antisera using ELISA or other immunoassay format for an additional cost. See the immunoassay section for more information.